Abstract
The signal recognition particle (SRP) receptor is a major player in the pathway of membrane protein biogenesis in all organisms. The receptor functions as a membrane-bound entity but very little is known about its targeting to the membrane. Here, we demonstrate in vivo that the Escherichia coli SRP receptor targets the membrane co-translationally. This requires emergence from the ribosome of the four-helix-long N-domain of the receptor, of which only helices 2-4 are required for co-translational membrane attachment. The results also suggest that the targeting might be regulated co-translationally. Taken together, our in vivo studies shed light on the biogenesis of the SRP receptor and its hypothetical role in targeting ribosomes to the E. coli membrane.
Keywords:
Co-translational targeting; FtsY; Membrane protein biogenesis; SecM; Translation intermediate.
© 2015. Published by The Company of Biologists Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Cell Membrane / genetics
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Cell Membrane / metabolism*
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Escherichia coli / chemistry
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Protein Biosynthesis*
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Protein Structure, Tertiary
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Protein Transport
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Receptors, Cytoplasmic and Nuclear / chemistry
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Receptors, Peptide / chemistry
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Receptors, Peptide / genetics
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Receptors, Peptide / metabolism*
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Ribosomes / genetics
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Ribosomes / metabolism
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Signal Recognition Particle / genetics
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Signal Recognition Particle / metabolism
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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Ffh protein, E coli
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FtsY protein, Bacteria
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Receptors, Cytoplasmic and Nuclear
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Receptors, Peptide
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Signal Recognition Particle
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signal peptide receptor