Proteinase mutants of Saccharomyces cerevisiae

Genetics. 1977 Jan;85(1):23-33. doi: 10.1093/genetics/85.1.23.

Abstract

Fifty-nine mutants with reduced ability to cleave the chymotrypsin substrate N-acetyl-DL-phenylalanine beta-naphthyl ester have been isolated in S. cerevisiae. All have reduced levels of one or more of the three well-characterized proteinases in yeast. All have reduced levels of proteinase C (carboxy-peptidase Y). These mutations define 16 complementation groups.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crosses, Genetic
  • Electrophoresis, Polyacrylamide Gel
  • Genes*
  • Genetic Linkage
  • Mutation
  • Naphthalenes
  • Peptide Hydrolases / metabolism*
  • Phenylalanine / analogs & derivatives
  • Phenylalanine / metabolism
  • Saccharomyces cerevisiae / enzymology*

Substances

  • Naphthalenes
  • N-acetylphenylalanine beta-naphthyl ester
  • Phenylalanine
  • Peptide Hydrolases