Abstract
Fifty-nine mutants with reduced ability to cleave the chymotrypsin substrate N-acetyl-DL-phenylalanine beta-naphthyl ester have been isolated in S. cerevisiae. All have reduced levels of one or more of the three well-characterized proteinases in yeast. All have reduced levels of proteinase C (carboxy-peptidase Y). These mutations define 16 complementation groups.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Crosses, Genetic
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Electrophoresis, Polyacrylamide Gel
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Genes*
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Genetic Linkage
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Mutation
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Naphthalenes
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Peptide Hydrolases / metabolism*
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Phenylalanine / analogs & derivatives
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Phenylalanine / metabolism
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Saccharomyces cerevisiae / enzymology*
Substances
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Naphthalenes
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N-acetylphenylalanine beta-naphthyl ester
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Phenylalanine
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Peptide Hydrolases