pH and the cAMP-dependent protein kinase mediate growth phase induction of the cytochrome c oxidase subunit VI gene, COX6, in Saccharomyces cerevisiae

Biochem Biophys Res Commun. 1995 Sep 25;214(3):1051-9. doi: 10.1006/bbrc.1995.2392.

Abstract

Respiratory adaptation in Saccharomyces cerevisiae is a complex genetic program that ensures ATP synthesis in a glucose-depleted environment. ATP is generated during respiration by the mitochondrial electron transport chain which is induced by respiratory adaptation. We have studied the terminal enzyme in mitochondrial electron transport, cytochrome c oxidase, from S. cerevisiae. Because subunits in this multisubunit enzyme are coordinately regulated, we have focused upon the well characterized subunit VI gene, COX6. In yeast, COX6 transcription is regulated by several factors thought to mediate respiratory adaptation including growth phase induction, oxygen dependence, and glucose repression. In the present study, we found that in addition to these known regulators, COX6 expression also depends upon pH and the cAMP-dependent protein kinase which may comprise additional factors signaling respiratory adaptation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Electron Transport Complex IV / biosynthesis*
  • Electron Transport Complex IV / genetics
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal*
  • Genotype
  • Hydrogen-Ion Concentration*
  • Kinetics
  • Macromolecular Substances
  • Oligonucleotide Probes
  • Recombinant Fusion Proteins / biosynthesis
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Transcription, Genetic
  • beta-Galactosidase / biosynthesis

Substances

  • Macromolecular Substances
  • Oligonucleotide Probes
  • Recombinant Fusion Proteins
  • Electron Transport Complex IV
  • Cyclic AMP-Dependent Protein Kinases
  • beta-Galactosidase