Abstract
We have isolated, sequenced, mapped and disrupted a novel gene, CCC1, from Saccharomyces cerevisiae. This gene displays non-allelic complementation of the Ca(2+)-sensitive phenotype conferred by the csg1 mutation. The ability of this gene, in two copies per cell, to reverse the csg1 defect suggests it may have a role in regulating Ca2+ homeostasis. The sequence of CCC1 indicates that it encodes a 322 amino acid, membrane-associated protein. The CCC1 gene is located on the right arm of chromosome XII.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Calcium / pharmacology*
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Cation Transport Proteins
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Chromosome Mapping
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Chromosomes, Fungal
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Fungal Proteins / chemistry
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Fungal Proteins / genetics*
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Fungal Proteins / physiology*
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Genes, Fungal*
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Genetic Complementation Test
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Molecular Sequence Data
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Phenotype
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Protein Structure, Tertiary
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
Substances
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CCC1 protein, S cerevisiae
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Cation Transport Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Calcium