Sequence, mapping and disruption of CCC1, a gene that cross-complements the Ca(2+)-sensitive phenotype of csg1 mutants

Yeast. 1994 Apr;10(4):515-21. doi: 10.1002/yea.320100411.

Abstract

We have isolated, sequenced, mapped and disrupted a novel gene, CCC1, from Saccharomyces cerevisiae. This gene displays non-allelic complementation of the Ca(2+)-sensitive phenotype conferred by the csg1 mutation. The ability of this gene, in two copies per cell, to reverse the csg1 defect suggests it may have a role in regulating Ca2+ homeostasis. The sequence of CCC1 indicates that it encodes a 322 amino acid, membrane-associated protein. The CCC1 gene is located on the right arm of chromosome XII.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Calcium / pharmacology*
  • Cation Transport Proteins
  • Chromosome Mapping
  • Chromosomes, Fungal
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Fungal Proteins / physiology*
  • Genes, Fungal*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Phenotype
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*

Substances

  • CCC1 protein, S cerevisiae
  • Cation Transport Proteins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Calcium

Associated data

  • GENBANK/L24112