The protein encoded by a growth arrest-specific gene (gas6) is a new member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade

Mol Cell Biol. 1993 Aug;13(8):4976-85. doi: 10.1128/mcb.13.8.4976-4985.1993.

Abstract

A set of growth arrest-specific genes (gas) whose expression is negatively regulated after serum induction has previously been described (C. Schneider, R. M. King, and L. Philipson, Cell 54:787-793, 1988). The detailed analysis of one of them, gas6, is reported here, gas6 mRNA (2.6 kb) is abundantly expressed in serum-starved (48 h in 0.5% fetal calf serum) NIH 3T3 cells but decreases dramatically after fetal calf serum or basic fibroblast growth factor stimulation. The human homolog of gas6 was also cloned and sequenced, revealing a high degree of homology and a similar pattern of expression in IMR90 human fibroblasts. Computer analysis of the protein encoded by murine and human gas6 cDNAs showed significant homology (43 and 44% amino acid identity, respectively) to human protein S, a negative coregulator in the blood coagulation pathway. By using an anti-human Gas6 monospecific affinity-purified antibody, we show that the biosynthetic level of human Gas6 fully reflects mRNA expression in IMR90 human fibroblasts. This finding thus defines a new member of vitamin K-dependent proteins that is expressed in many human and mouse tissues and may be involved in the regulation of a protease cascade relevant in growth regulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Base Sequence
  • Blood Coagulation
  • Cell Division*
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression Regulation
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Mice
  • Molecular Sequence Data
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Vitamin K

Substances

  • Intercellular Signaling Peptides and Proteins
  • Proteins
  • RNA, Messenger
  • growth arrest-specific protein 6
  • Vitamin K
  • DNA

Associated data

  • GENBANK/L13720
  • GENBANK/X59846