Sequencing and characterization of the sdaB gene from Escherichia coli K-12

Eur J Biochem. 1993 Mar 15;212(3):777-84. doi: 10.1111/j.1432-1033.1993.tb17718.x.

Abstract

The sdaB gene which codes for the second L-serine deaminase (L-SD) of Escherichia coli K-12 has been sequenced and shown to be very similar to the sdaA gene which codes for the first L-serine deaminase. sdaB is transcribed in rich medium, particularly in the absence of glucose, and is under the control of catabolite activator protein. A mutation which established expression of the sdaB gene and synthesis of L-serine deaminase 2 in minimal medium has been demonstrated to result in a change in the ribosome-binding site of the sdaB gene.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Chromosomes, Bacterial
  • Cloning, Molecular / methods
  • Codon / genetics
  • Cyclic AMP Receptor Protein / metabolism
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Genotype
  • Isoenzymes / genetics*
  • Kinetics
  • L-Serine Dehydratase / genetics*
  • L-Serine Dehydratase / metabolism
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Oligodeoxyribonucleotides
  • Open Reading Frames
  • Plasmids
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / metabolism
  • Ribosomes / metabolism
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Serine / metabolism
  • Threonine / metabolism
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Codon
  • Cyclic AMP Receptor Protein
  • DNA, Bacterial
  • Isoenzymes
  • Oligodeoxyribonucleotides
  • Recombinant Fusion Proteins
  • Threonine
  • Serine
  • beta-Galactosidase
  • L-Serine Dehydratase

Associated data

  • GENBANK/L07763
  • GENBANK/L09679
  • GENBANK/L09680
  • GENBANK/L09681
  • GENBANK/L09682
  • GENBANK/L09683
  • GENBANK/X57950
  • GENBANK/X70793
  • GENBANK/X70999
  • GENBANK/X71000