Abstract
The sdaB gene which codes for the second L-serine deaminase (L-SD) of Escherichia coli K-12 has been sequenced and shown to be very similar to the sdaA gene which codes for the first L-serine deaminase. sdaB is transcribed in rich medium, particularly in the absence of glucose, and is under the control of catabolite activator protein. A mutation which established expression of the sdaB gene and synthesis of L-serine deaminase 2 in minimal medium has been demonstrated to result in a change in the ribosome-binding site of the sdaB gene.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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Chromosomes, Bacterial
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Cloning, Molecular / methods
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Codon / genetics
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Cyclic AMP Receptor Protein / metabolism
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Genes, Bacterial*
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Genotype
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Isoenzymes / genetics*
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Kinetics
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L-Serine Dehydratase / genetics*
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L-Serine Dehydratase / metabolism
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Oligodeoxyribonucleotides
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Open Reading Frames
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Plasmids
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Promoter Regions, Genetic
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Recombinant Fusion Proteins / metabolism
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Ribosomes / metabolism
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Serine / metabolism
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Threonine / metabolism
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Codon
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Cyclic AMP Receptor Protein
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DNA, Bacterial
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Isoenzymes
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Oligodeoxyribonucleotides
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Recombinant Fusion Proteins
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Threonine
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Serine
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beta-Galactosidase
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L-Serine Dehydratase
Associated data
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GENBANK/L07763
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GENBANK/L09679
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GENBANK/L09680
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GENBANK/L09681
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GENBANK/L09682
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GENBANK/L09683
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GENBANK/X57950
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GENBANK/X70793
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GENBANK/X70999
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GENBANK/X71000