Abstract
The gene spy from Escherichia coli has been cloned and sequenced. It encodes a precursor of a so far unknown 139-residue, rather basic periplasmic protein. It was not detectable immunologically in intact cells but was produced abundantly in spheroplasts. It could be a stress protein specific for spheroplasting.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / analysis
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Base Sequence
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Cytoplasm / chemistry
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli / ultrastructure
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Escherichia coli Proteins*
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Genes, Bacterial
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Microscopy, Electron
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Molecular Sequence Data
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Mutation
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Periplasmic Proteins*
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Spheroplasts / metabolism*
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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Periplasmic Proteins
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Spy protein, E coli