Adam2 a disintegrin and metallopeptidase domain 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Adam2provided by MGI
Official Full Name: a disintegrin and metallopeptidase domain 2provided by MGI
Primary source: MGI:MGI:1340894
See related: Ensembl:ENSMUSG00000022039 AllianceGenome:MGI:1340894
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ftnb; Ph30-beta
Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is predominantly expressed in the epididymis, where the encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Male mice lacking the encoded protein are infertile and exhibit multiple defects in reproduction. [provided by RefSeq, May 2016]
Expression: Restricted expression toward testis adult (RPKM 65.0) See more
Orthologs: human all
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Genomic context

Location:: 14 D1; 14 34.36 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (66264778..66315300, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (66027322..66077851, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer.
Title: In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer.
Characterization of promoter and repressor regions that regulate the transcription of the mouse Adam2 gene.
Title: Identification and characterization of promoter and regulatory regions for mouse Adam2 gene expression.
data indicate that ITGA9-ITGB7 functions as an ADAM binding partner in certain cellular contexts, with implications for mammalian fertilization and integrin function
Title: ADAM2 interactions with mouse eggs and cell lines expressing α4/α9 (ITGA4/ITGA9) integrins: implications for integrin-based adhesion and fertilization.
we report that Adam2 and Adam3 knockout sperm have severely impaired sperm aggregation and that this defect is not restored over time during in vitro cultivation.
Title: Impaired sperm aggregation in Adam2 and Adam3 null mice.
ADAM2 contributes to migration from the subventricular zone along the rostral migratory stream, possibly through cell-cell interactions that mediate the rapid migration of the neuroblasts to their endpoint.
Title: ADAM2 promotes migration of neuroblasts in the rostral migratory stream to the olfactory bulb.
Results suggest that association with ADAM2 is a key element for stability of ADAM3 in epididymal sperm, and the presence of the ADAM2-ADAM3 complex in sperm also suggests a potential role of ADAM2 with ADAM3 in sperm binding to the egg zona pellucida.
Title: Identification of an ADAM2-ADAM3 complex on the surface of mouse testicular germ cells and cauda epididymal sperm.
ADAM1a/ADAM2 Fertilin complex has roles in expression of ADAM3 on the cell surface of mouse sperm
Title: Possible function of the ADAM1a/ADAM2 Fertilin complex in the appearance of ADAM3 on the sperm surface.
ADAM2 is involved in protein trafficking during sperm development
Title: Defects in secretory pathway trafficking during sperm development in Adam2 knockout mice.
Because fertilin beta is absent from calmegin-deficient mice, the role of fertilin beta in sperm-EPM interaction needs to be reevaluated.
Title: Sperm from the calmegin-deficient mouse have normal abilities for binding and fusion to the egg plasma membrane.
These results suggest that mouse ADAM1b/ADAM2 fertilin may play a crucial role not in the sperm/egg fusion but in the appearance of these two ADAMs on the sperm surface.
Title: Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

mAdam2 (e-PCR)
- UniSTS:514571

AI323749 (e-PCR)
- UniSTS:186007

Adam2 (e-PCR), detects polymorphism
- UniSTS:141027

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within adult behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in binding of sperm to zona pellucida	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell adhesion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male gonad development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within visual learning	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in acrosomal vesicle	ISO Inferred from Sequence Orthology more info
is_active_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: disintegrin and metalloproteinase domain-containing protein 2

Names: a disintegrin and metalloprotease domain 2 (fertilin beta); fertilin subunit beta

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009618.4 → NP_033748.2 disintegrin and metalloproteinase domain-containing protein 2 preproprotein

See identical proteins and their annotated locations for NP_033748.2

Status: REVIEWED

Source sequence(s)

AC126272, AC126444

Consensus CDS

CCDS36959.1

UniProtKB/Swiss-Prot

Q60718, Q60814, Q9D4G3, Q9QWJ0

UniProtKB/TrEMBL

Q3TTP6

Related

ENSMUSP00000022618.6, ENSMUST00000022618.6

Conserved Domains (5) summary

smart00608
Location:475 → 610

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:185 → 379

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:398 → 469

Disintegrin; Disintegrin

pfam01421
Location:184 → 381

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 146

Pep_M12B_propep; Reprolysin family propeptide

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

66264778..66315300 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006518439.4 → XP_006518502.1 disintegrin and metalloproteinase domain-containing protein 2 isoform X1

UniProtKB/TrEMBL

Q3TTP6

Conserved Domains (4) summary

smart00608
Location:451 → 586

ACR; ADAM Cysteine-Rich Domain

pfam00200
Location:374 → 445

Disintegrin

pfam01421
Location:184 → 357

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:32 → 147

Pep_M12B_propep; Reprolysin family propeptide