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    Clnk cytokine-dependent hematopoietic cell linker [ Mus musculus (house mouse) ]

    Gene ID: 27278, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Clnkprovided by MGI
    Official Full Name
    cytokine-dependent hematopoietic cell linkerprovided by MGI
    Primary source
    MGI:MGI:1351468
    See related
    Ensembl:ENSMUSG00000039315 AllianceGenome:MGI:1351468
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    MIST
    Summary
    Involved in several processes, including mast cell degranulation; negative regulation of natural killer cell activation; and positive regulation of natural killer cell cytokine production. Acts upstream of or within cell surface receptor protein tyrosine kinase signaling pathway. Located in mast cell granule. Part of protein-containing complex. Orthologous to human CLNK (cytokine dependent hematopoietic cell linker). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Low expression observed in reference dataset See more
    Orthologs
    human all
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    Genomic context

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    See Clnk in Genome Data Viewer
    Location:
    5 B3; 5 20.81 cM
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (38858951..39034201, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (38705588..38876858, complement)

    Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_12927 Neighboring gene zinc finger protein 518B Neighboring gene predicted gene, 22779 Neighboring gene STARR-seq mESC enhancer starr_12931 Neighboring gene STARR-seq mESC enhancer starr_12932 Neighboring gene STARR-seq mESC enhancer starr_12933 Neighboring gene STARR-seq mESC enhancer starr_12935 Neighboring gene predicted gene, 46937 Neighboring gene STARR-positive B cell enhancer mm9_chr5:39547024-39547325 Neighboring gene STARR-seq mESC enhancer starr_12936 Neighboring gene predicted gene, 40293

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells. Utting O, et al. Mol Cell Biol, 2004 Jul. PMID 15199160, Free PMC Article
    2. Distinct regulatory functions of SLP-76 and MIST in NK cell cytotoxicity and IFN-gamma production. Hidano S, et al. Int Immunol, 2008 Mar. PMID 18203684
    3. Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5. Sasanuma H, et al. Biochem Biophys Res Commun, 2004 Aug 13. PMID 15358227
    4. Dual function for the adaptor MIST in IFN-gamma production by NK and CD4+NKT cells regulated by the Src kinase Fgr. Sasanuma H, et al. Blood, 2006 May 1. PMID 16439675
    5. Clnk, a novel SLP-76-related adaptor molecule expressed in cytokine-stimulated hemopoietic cells. Cao MY, et al. J Exp Med, 1999 Nov 15. PMID 10562326, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Clnk is required for TNF-alpha -induced cell death in L929 cells.
      Title: Clnk plays a role in TNF-alpha-induced cell death in murine fibrosarcoma cell line L929.
    2. Clnk is dispensable for normal differentiation and function of T cells, mast cells, and NK cells. Hence, unlike its relatives, Clnk is not essential for normal immune functions
      Title: Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.
    3. STAT5 is involved in cytokine-mediated up-regulation of MIST gene expression, in collaboration with other lineage-specific transcription factors that promote basal MIST expression in mast cells.
      Title: Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5.
    4. interaction of MIST with Fgr, mediated by the C-terminal proline-rich region of MIST and the SH3 domain of Fgr, was required for the suppression of NK-cell receptor-induced IFN-gamma production
      Title: Dual function for the adaptor MIST in IFN-gamma production by NK and CD4+NKT cells regulated by the Src kinase Fgr.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (4) 
    • Targeted (3)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC159342

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein-containing complex binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell surface receptor protein tyrosine kinase signaling pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within cell surface receptor protein tyrosine kinase signaling pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cell surface receptor protein tyrosine kinase signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in immune response NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in intracellular signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in intracellular signal transduction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mast cell degranulation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of natural killer cell activation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of natural killer cell cytokine production IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mast cell granule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    cytokine-dependent hematopoietic cell linker
    Names
    mast cell immunoreceptor signal transducer

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_013748.3 → NP_038776.3  cytokine-dependent hematopoietic cell linker

      See identical proteins and their annotated locations for NP_038776.3

      Status: PROVISIONAL

      Source sequence(s)
      AF187819
      Consensus CDS
      CCDS51486.1
      UniProtKB/Swiss-Prot
      Q8C479, Q9JMJ3, Q9QZE2
      Related
      ENSMUSP00000128473.2, ENSMUST00000169819.5
      Conserved Domains (1) summary
      cl15255
      Location:300 → 416
      SH2; Src homology 2 (SH2) domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000071.7 Reference GRCm39 C57BL/6J

      Range
      38858951..39034201 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011240734.2 → XP_011239036.1  cytokine-dependent hematopoietic cell linker isoform X2

      Conserved Domains (1) summary
      cl15255
      Location:276 → 392
      SH2; Src homology 2 (SH2) domain

    2. XM_006503974.3 → XP_006504037.1  cytokine-dependent hematopoietic cell linker isoform X1

      See identical proteins and their annotated locations for XP_006504037.1

      UniProtKB/Swiss-Prot
      Q8C479, Q9JMJ3, Q9QZE2
      Conserved Domains (1) summary
      cl15255
      Location:300 → 416
      SH2; Src homology 2 (SH2) domain

    3. XM_011240735.2 → XP_011239037.1  cytokine-dependent hematopoietic cell linker isoform X3

      Conserved Domains (1) summary
      cl15255
      Location:218 → 334
      SH2; Src homology 2 (SH2) domain

    4. XM_006503977.5 → XP_006504040.1  cytokine-dependent hematopoietic cell linker isoform X4

      Conserved Domains (1) summary
      cl15255
      Location:100 → 216
      SH2; Src homology 2 (SH2) domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9QZE2.1 GenPept UniProtKB/Swiss-Prot:Q9QZE2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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