pbl pebble [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: pblprovided by FlyBase
Official Full Name: pebbleprovided by FlyBase
Primary source: FLYBASE:FBgn0003041
Locus tag: Dmel_CG8114
See related: AllianceGenome:FB:FBgn0003041
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0083/20; 0293/09; 0542/03; anon-WO0172774.22; anon-WO0172774.25; CG8114; Dmel\CG8114; Ect-2; ECT2; GEF; l(3)09645; Pbl; PBL; Pbl/Ect2; Peb
Summary: Enables several functions, including guanyl-nucleotide exchange factor activity; semaphorin receptor binding activity; and small GTPase binding activity. Involved in several processes, including cytoskeleton-dependent cytokinesis; defasciculation of motor neuron axon; and embryonic morphogenesis. Located in cell cortex; cleavage furrow; and nucleus. Is expressed in several structures, including adult brain cell body rind; epithelium; ganglia; germ layer; and gut section. Orthologous to human ECT2 (epithelial cell transforming 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 66A18-66A19; 3-21 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (7896109..7911954, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (7889209..7905054, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The PDZ domain binding motif found in the most C-terminal region of the Sema-1a ICD is necessary for interaction with Vari, but not Cher, indicative of distinct binding modalities. Pbl/Sema-1a-mediated repulsive guidance is potentiated by both vari and cher Genetic analyses further suggest that scaffolding functions of Vari and Cher play an important role in Pbl-mediated Sema-1a reverse signaling.
Title: Varicose and cheerio collaborate with pebble to mediate semaphorin-1a reverse signaling in Drosophila.
RhoGEF2, Pbl, RhoGEF3, and Tum exhibit discrete localization patterns and are required for cell wound repair.
Title: Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.
Both Pbl and ECT2 repress Wg/Wnt target gene expression in cultured Drosophila and human cells.
Title: Pebble/ECT2 RhoGEF negatively regulates the Wingless/Wnt signaling pathway.
Reduced cytokinesis may be responsible for the left-right defects in the hindgut of embryos homozygous for pbl.
Title: Reduced cell number in the hindgut epithelium disrupts hindgut left-right asymmetry in a mutant of pebble, encoding a RhoGEF, in Drosophila embryos.
early Drosophila embryogenesis is primed to form furrows at either overlapping astral microtubules or the central spindle; the shift to the latter is driven by a corresponding shift from RhoGEF2 to Pebble in controlling furrow formation.
Title: RhoGEF and positioning of rappaport-like furrows in the early Drosophila embryo.
Study shows that Sna represses transcription of pbl in the mesoderm primordium of D. melanogaster via one or more Sna-binding sites, which are conserved among species of the Drosophila genus, but not in the mosquito, correlating with the different modes of gastrulation in the different genuses.
Title: Snail-dependent repression of the RhoGEF pebble is required for gastrulation consistency in Drosophila melanogaster.
interaction of the PH-domain of Pbl with PtdIns(4,5)P2 helps localise it to the plasma membrane which is important for mesoderm migration.
Title: Regulation of Drosophila mesoderm migration by phosphoinositides and the PH domain of the Rho GTP exchange factor Pebble.
This study demonistrated that The Control of semaphorin-1a-mediated reverse signaling by opposing pebble and RhoGAPp190 functions in drosophila development.
Title: The Control of semaphorin-1a-mediated reverse signaling by opposing pebble and RhoGAPp190 functions in drosophila.
gain-of-function and rescue experiments suggest an important regulatory role of the C-terminal tail of Pbl for the selective activation of Rho1-versus Rac-dependent pathways
Title: Regulation of the Rac GTPase pathway by the multifunctional Rho GEF Pebble is essential for mesoderm migration in the Drosophila gastrula.
A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis.
Title: A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables phosphatidylinositol phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables semaphorin receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Malpighian tubule morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defasciculation of motor neuron axon	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fibroblast growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in maintenance of protein location in cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiosis I cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiosis II cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mesoderm migration involved in gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mesodermal cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of myotube cell migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of GTPase activity	IEA Inferred from Electronic Annotation more info
involved_in regulation of axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytokinesis, actomyosin contractile ring assembly	IEA Inferred from Electronic Annotation more info
involved_in response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in wound healing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: pebble

Names: CG8114-PA; CG8114-PB; CG8114-PC; CG8114-PD; CG8114-PE; CG8114-PF; pbl-PA; pbl-PB; pbl-PC; pbl-PD; pbl-PE; pbl-PF; pebble RhoGEF

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

7896109..7911954 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_168241.3 → NP_729306.1 pebble, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_729306.1

Status: REVIEWED

UniProtKB/TrEMBL

Q58CL4, Q9U7D8, Q9VSB7

Related

FBpp0076486

Conserved Domains (4) summary

cd01229
Location:565 → 728

PH_Ect2; Epithelial cell transforming 2 (Ect2) pleckstrin homology (PH) domain

cd17732
Location:209 → 287

BRCT_Ect2_rpt2; second BRCT domain of epithelial cell-transforming sequence 2 protein (ECT2) and similar proteins

pfam00621
Location:394 → 573

RhoGEF; RhoGEF domain

cl00038
Location:114 → 189

BRCT; C-terminal domain of the breast cancer suppressor protein (BRCA1) and related domains
NM_079241.4 → NP_523965.2 pebble, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523965.2

Status: REVIEWED

UniProtKB/TrEMBL

Q8IQ97

Related

FBpp0076487

Conserved Domains (3) summary

cd01229
Location:1023 → 1186

PH_Ect2; Epithelial cell transforming 2 (Ect2) pleckstrin homology (PH) domain

pfam00621
Location:852 → 1032

RhoGEF; RhoGEF domain

pfam12738
Location:117 → 180

PTCB-BRCT; twin BRCT domain
NM_001104070.2 → NP_001097540.1 pebble, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097540.1

Status: REVIEWED

UniProtKB/TrEMBL

A8JNN0, Q58CL4

Conserved Domains (4) summary

smart00292
Location:254 → 323

BRCT; breast cancer carboxy-terminal domain

cd01229
Location:612 → 775

PH_Ect2; Epithelial cell transforming 2 (Ect2) pleckstrin homology (PH) domain

pfam00621
Location:441 → 621

RhoGEF; RhoGEF domain

pfam12738
Location:164 → 227

PTCB-BRCT; twin BRCT domain
NM_168242.3 → NP_729307.1 pebble, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_729307.1

Status: REVIEWED

UniProtKB/TrEMBL

Q5U192, Q8IQ96

Related

FBpp0076488

Conserved Domains (4) summary

smart00292
Location:136 → 205

BRCT; breast cancer carboxy-terminal domain

cd01229
Location:494 → 657

PH_Ect2; Epithelial cell transforming 2 (Ect2) pleckstrin homology (PH) domain

pfam00621
Location:323 → 503

RhoGEF; RhoGEF domain

pfam12738
Location:46 → 109

PTCB-BRCT; twin BRCT domain
NM_168243.3 → NP_729308.1 pebble, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_729308.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8IQ95

Related

FBpp0076489

Conserved Domains (3) summary

cd01229
Location:952 → 1115

PH_Ect2; Epithelial cell transforming 2 (Ect2) pleckstrin homology (PH) domain

pfam00621
Location:781 → 961

RhoGEF; RhoGEF domain

pfam12738
Location:46 → 109

PTCB-BRCT; twin BRCT domain
NM_001259732.1 → NP_001246661.1 pebble, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246661.1

Status: REVIEWED

UniProtKB/TrEMBL

D2NUE8

Conserved Domains (2) summary

cd01229
Location:279 → 442

PH_Ect2; Epithelial cell transforming 2 (Ect2) pleckstrin homology (PH) domain

pfam00621
Location:108 → 287

RhoGEF; RhoGEF domain