DNF2 aminophospholipid-translocating P4-type ATPase DNF2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DNF2
Official Full Name: aminophospholipid-translocating P4-type ATPase DNF2
Primary source: SGD:S000002500
Locus tag: YDR093W
See related: AllianceGenome:SGD:S000002500; FungiDB:YDR093W; VEuPathDB:YDR093W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables glycosylceramide flippase activity and phosphatidylserine flippase activity. Involved in endocytosis; establishment or maintenance of cell polarity; and lipid translocation. Located in mating projection tip membrane. Is active in plasma membrane. Used to study progressive familial intrahepatic cholestasis. Orthologous to several human genes including ATP10A (ATPase phospholipid transporting 10A (putative)) and ATP10D (ATPase phospholipid transporting 10D (putative)). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DNF2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (631282..636120)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lipid flippases in polarized growth.
Title: Lipid flippases in polarized growth.
Exofacial membrane composition and lipid metabolism regulates plasma membrane P4-ATPase substrate specificity.
Title: Exofacial membrane composition and lipid metabolism regulates plasma membrane P4-ATPase substrate specificity.
Transport mechanism of P4 ATPase phosphatidylcholine flippases.
Title: Transport mechanism of P4 ATPase phosphatidylcholine flippases.
An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast.
Title: An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast.
Data show that Lem3-Dnf1/Dnf2 complex regulates the polar Cdc42 dynamics and phosphatidylserine distribution.
Title: Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.
aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae
Title: The putative aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae.
The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled intramembrane lipid transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATPase-coupled intramembrane lipid transporter activity	IEA Inferred from Electronic Annotation more info
enables aminophospholipid flippase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables aminophospholipid flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycosylceramide flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylcholine floppase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylethanolamine flippase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylserine flippase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylserine flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylserine floppase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aminophospholipid translocation	IEA Inferred from Electronic Annotation more info
involved_in ceramide translocation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
involved_in phospholipid translocation	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipid translocation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phospholipid translocation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid transport	IEA Inferred from Electronic Annotation more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell periphery	HDA more info	PubMed
located_in cellular bud neck	HDA more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in mating projection tip membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: aminophospholipid-translocating P4-type ATPase DNF2

NP_010378.1

EC 7.6.2.1

Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

631282..636120

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180401.1 → NP_010378.1 TPA: aminophospholipid-translocating P4-type ATPase DNF2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010378.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VS80, Q12675

UniProtKB/TrEMBL

C7GVP3, C8Z511

Conserved Domains (1) summary

TIGR01652
Location:229 → 1456

ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase