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    STE5 Ste5p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851680, updated on 9-Dec-2024

    Summary

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    Official Symbol
    STE5
    Official Full Name
    Ste5p
    Primary source
    SGD:S000002510
    Locus tag
    YDR103W
    See related
    AllianceGenome:SGD:S000002510; FungiDB:YDR103W; VEuPathDB:YDR103W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    HMD3; NUL3
    Summary
    Enables G-protein beta-subunit binding activity; MAP-kinase scaffold activity; and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including invasive growth in response to glucose limitation; negative regulation of MAPK cascade; and pheromone-dependent signal transduction involved in conjugation with cellular fusion. Located in cytoplasm; mating projection tip membrane; and nucleus. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See STE5 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (658350..661103)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene putative hydrolase Neighboring gene uncharacterized protein Neighboring gene Spo71p Neighboring gene Tms1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Quantitative effect of scaffold abundance on signal propagation. Chapman SA, et al. Mol Syst Biol, 2009. PMID 19888208, Free PMC Article
    2. Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components. Wang Y, et al. Mol Biol Cell, 2003 Jun. PMID 12808050, Free PMC Article
    3. Dynamics and organization of MAP kinase signal pathways. Errede B, et al. Mol Reprod Dev, 1995 Dec. PMID 8607979
    4. Dimerization of Ste5, a mitogen-activated protein kinase cascade scaffold protein, is required for signal transduction. Yablonski D, et al. Proc Natl Acad Sci U S A, 1996 Nov 26. PMID 8943027, Free PMC Article
    5. Function of the MAPK scaffold protein, Ste5, requires a cryptic PH domain. Garrenton LS, et al. Genes Dev, 2006 Jul 15. PMID 16847350, Free PMC Article

    See all (152) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Effects of HSP70 chaperones Ssa1 and Ssa2 on Ste5 scaffold and the mating mitogen-activated protein kinase (MAPK) pathway in Saccharomyces cerevisiae.
      Title: Effects of HSP70 chaperones Ssa1 and Ssa2 on Ste5 scaffold and the mating mitogen-activated protein kinase (MAPK) pathway in Saccharomyces cerevisiae.
    2. Pkc1 triggers dispersal of Ste5 from mating projections upon mechanically induced stress and during cell-cell fusion, leading to inhibition of the MAPK Fus3.
      Title: Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5.
    3. phosphorylation of the yeast Ggamma subunit (Ste18), together with Fus3 docking on Ste5, controls the rate and stability of Ste5/plasma membrane association.
      Title: Negative Feedback Phosphorylation of Gγ Subunit Ste18 and the Ste5 Scaffold Synergistically Regulates MAPK Activation in Yeast.
    4. CDK and MAPK modify shared sites, which are most extensively phosphorylated when both kinases are active and able to bind their docking sites on Ste5. synergistically regulating signaling dynamics.
      Title: CDK and MAPK Synergistically Regulate Signaling Dynamics via a Shared Multi-site Phosphorylation Region on the Scaffold Protein Ste5.
    5. defining regions of Ste5 that mediate nuclear export
      Title: Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export.
    6. The Ras-binding-domain-like region in the Ste11 protein that is required specifically for the kinase to function with Ste5 in the mating pathway
      Title: Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling.
    7. Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway.
      Title: Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway.
    8. findings show that the scaffold protein Ste5, which organizes the yeast mating mitogen-activated protein kinase pathway, does not use sequestration to prevent misactivation of the mating response; Ste5 appears to use a conformation mechanism
      Title: Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation.
    9. The signaling proteins Ste5 and Ste20 have recognition sequences, or "docking" sites, for G1/S (interphase) cyclins. These docking sites bind preferentially to the cyclin Cln2, and strongly enhance Cln2-driven in vivo substrate phosphorylation.
      Title: Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes.
    10. a switch-like dissociation of Fus3 from Ste5 that is necessary to generate the switch-like mating response
      Title: The scaffold protein Ste5 directly controls a switch-like mating decision in yeast.
    Submit: New GeneRIF Correction See all GeneRIFs (19)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables G-protein beta-subunit binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables MAP-kinase scaffold activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables MAP-kinase scaffold activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables kinase binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphatidylinositol-4,5-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in MAPK cascade IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in invasive growth in response to glucose limitation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of MAPK cascade IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to pheromone IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mating projection tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mating projection tip membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Ste5p
    NP_010388.1
    • Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      658350..661103
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180411.1 → NP_010388.1  TPA: Ste5p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010388.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VS89, P32917
      UniProtKB/TrEMBL
      B3LGH2, B5VG01, C8Z519
      Conserved Domains (3) summary
      pfam11610
      Location:287 → 316
      Ste5; Scaffold protein Ste5, Fus3-binding region
      pfam12194
      Location:594 → 775
      Ste5_C; Protein kinase Fus3-binding
      cl17238
      Location:176 → 207
      RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32917.2

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