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    CSE4 centromeric DNA-binding histone H3-like protein CSE4 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853817, updated on 17-Dec-2024

    Summary

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    Official Symbol
    CSE4
    Official Full Name
    centromeric DNA-binding histone H3-like protein CSE4
    Primary source
    SGD:S000001532
    Locus tag
    YKL049C
    See related
    AllianceGenome:SGD:S000001532; FungiDB:YKL049C; VEuPathDB:YKL049C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    CSL2
    Summary
    Enables centromeric DNA binding activity. Involved in 2-micrometer plasmid partitioning; chromosome organization; and protein localization to CENP-A containing chromatin. Located in 2-micrometer circle DNA; condensed chromosome, centromeric region; and peroxisome. Part of CENP-A containing nucleosome. Is active in kinetochore. Used to study cancer; male infertility; and oligospermia. Human ortholog(s) of this gene implicated in autoimmune disease. Orthologous to human CENPA (centromere protein A). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See CSE4 in Genome Data Viewer
    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (346075..346764, complement)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Sfk1p Neighboring gene Lpx2p Neighboring gene serine/threonine protein kinase ELM1 Neighboring gene Anr2p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Cell-cycle-coupled structural oscillation of centromeric nucleosomes in yeast. Shivaraju M, et al. Cell, 2012 Jul 20. PMID 22817893, Free PMC Article
    2. Cryoelectron Microscopy Structure of a Yeast Centromeric Nucleosome at 2.7 Å Resolution. Migl D, et al. Structure, 2020 Mar 3. PMID 32004465, Free PMC Article
    3. Function and assembly of DNA looping, clustering, and microtubule attachment complexes within a eukaryotic kinetochore. Anderson M, et al. Mol Biol Cell, 2009 Oct. PMID 19656849, Free PMC Article
    4. Histone H3-variant Cse4-induced positive DNA supercoiling in the yeast plasmid has implications for a plasmid origin of a chromosome centromere. Huang CC, et al. Proc Natl Acad Sci U S A, 2011 Aug 16. PMID 21807992, Free PMC Article
    5. An asymmetric centromeric nucleosome. Ichikawa Y, et al. Elife, 2018 Aug 23. PMID 30136924, Free PMC Article

    See all (179) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Interaction of histone H4 with Cse4 facilitates conformational changes in Cse4 for its sumoylation and mislocalization.
      Title: Interaction of histone H4 with Cse4 facilitates conformational changes in Cse4 for its sumoylation and mislocalization.
    2. Recognition of centromere-specific histone Cse4 by the inner kinetochore Okp1-Ame1 complex.
      Title: Recognition of centromere-specific histone Cse4 by the inner kinetochore Okp1-Ame1 complex.
    3. Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.
      Title: Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.
    4. Cdc48Ufd1/Npl4 segregase removes mislocalized centromeric histone H3 variant CENP-A from non-centromeric chromatin.
      Title: Cdc48Ufd1/Npl4 segregase removes mislocalized centromeric histone H3 variant CENP-A from non-centromeric chromatin.
    5. Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.
      Title: Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.
    6. Reduced gene dosage of histone H4 prevents CENP-A mislocalization and chromosomal instability in Saccharomyces cerevisiae.
      Title: Reduced gene dosage of histone H4 prevents CENP-A mislocalization and chromosomal instability in Saccharomyces cerevisiae.
    7. Transcription destabilizes centromere function.
      Title: Transcription destabilizes centromere function.
    8. Cell-cycle phospho-regulation of the kinetochore.
      Title: Cell-cycle phospho-regulation of the kinetochore.
    9. Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-Terminal Sumoylation.
      Title: Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-Terminal Sumoylation.
    10. Study revealed that two essential Skp, Cullin, F-box (SCF) ubiquitin ligases defined by the F-box proteins Met30 and Cdc4 cooperatively regulate Cse4 (S. cerevisiae homolog of human centromere protein-A (CENP-A)) proteolysis under normal physiological conditions. Results suggest SCF-Met30/Cdc4-mediated proteolysis of Cse4 is one of the major mechanisms that prevents stable maintenance of Cse4 at non-centromeric regions.
      Title: Skp, Cullin, F-box (SCF)-Met30 and SCF-Cdc4-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A for Chromosomal Stability in Budding Yeast.
    Submit: New GeneRIF Correction See all GeneRIFs (37)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables centromeric DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein heterodimerization activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables structural constituent of chromatin IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in 2-micrometer plasmid partitioning IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in kinetochore assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic cell cycle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mitotic sister chromatid segregation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein localization to CENP-A containing chromatin IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in 2-micrometer circle DNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of CENP-A containing nucleosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, centromeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in condensed chromosome, centromeric region IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in condensed chromosome, centromeric region IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    is_active_in kinetochore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of nucleosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in peroxisome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    centromeric DNA-binding histone H3-like protein CSE4
    NP_012875.2
    • Centromeric histone H3-like protein; associates with promoters, accessible chromatin and RNAPII-bound regions; centromeric association promotes kinetochore assembly and Sgo1p association with CEN chromatin; Ipl1p-dependent phosphorylation destabilizes defective kinetochores promoting biorientation; phosphorylation by Cdc7p maintains Cse4p at kinetochores and promotes chromosome segregation; R37 methylation regulates kinetochore function and chromosome segregation; complements human homolog CENPA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      346075..346764 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179615.1 → NP_012875.2  TPA: centromeric DNA-binding histone H3-like protein CSE4 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012875.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXN8, P36012
      UniProtKB/TrEMBL
      A6ZZR7, B3LR40, B5VMA8, C7GP25, C8ZCC3, G2WHY3, N1P2E0
      Conserved Domains (1) summary
      smart00428
      Location:124 → 229
      H3; Histone H3
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P36012.2

    Gene LinkOut

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