LCB4 sphinganine kinase LCB4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: LCB4
Official Full Name: sphinganine kinase LCB4
Primary source: SGD:S000005697
Locus tag: YOR171C
See related: AllianceGenome:SGD:S000005697; FungiDB:YOR171C; VEuPathDB:YOR171C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables D-erythro-sphingosine kinase activity. Involved in calcium-mediated signaling and sphingolipid metabolic process. Located in Golgi apparatus; cortical endoplasmic reticulum; and plasma membrane. Orthologous to human SPHK2 (sphingosine kinase 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See LCB4 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (652010..653884, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lcb4p is post-translationally modified by phosphorylation by Pho85 and degraded via the ubiquitin-dependent pathway
Title: Phosphorylation by Pho85 cyclin-dependent kinase acts as a signal for the down-regulation of the yeast sphingoid long-chain base kinase Lcb4 during the stationary phase.
The loss of palmitoylation on Lcb4 caused several effects, including mislocalization of the protein to the cytosol.
Title: Long-chain base kinase Lcb4 Is anchored to the membrane through its palmitoylation by Akr1.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables D-erythro-sphingosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables D-erythro-sphingosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables D-erythro-sphingosine kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables sphingosine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sphingolipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in sphingolipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in sphingosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in cell periphery	HDA more info	PubMed
located_in cortical endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in late endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sphinganine kinase LCB4

NP_014814.1

EC 2.7.1.91

Sphingoid long-chain base kinase; responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes; LCB4 has a paralog, LCB5, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

652010..653884 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183590.1 → NP_014814.1 TPA: sphinganine kinase LCB4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014814.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2M7, Q12246

UniProtKB/TrEMBL

B5VS55, C7GN55, C8ZGT2

Conserved Domains (1) summary

COG1597
Location:225 → 540

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]