RNR3 ribonucleotide-diphosphate reductase subunit RNR3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RNR3
Official Full Name: ribonucleotide-diphosphate reductase subunit RNR3
Primary source: SGD:S000001328
Locus tag: YIL066C
See related: AllianceGenome:SGD:S000001328; FungiDB:YIL066C; VEuPathDB:YIL066C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DIN1; RIR3
Summary: Contributes to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor. Involved in deoxyribonucleotide biosynthetic process. Located in cytoplasm. Human ortholog(s) of this gene implicated in chronic progressive external ophthalmoplegia. Orthologous to human RRM1 (ribonucleotide reductase catalytic subunit M1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RNR3 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (238099..240708, complement)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The gene encoding a large subunit of ribonucleotide reductase, RNR3, is regulated by ISW2 and SWI/SNF, complexes that repress and activate transcription, respectively. Here, we studied the functional interactions of these two complexes at RNR3.
Title: A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression.
RNR3 is dependent upon both TFIID and SAGA, two complexes that deliver TATA-binding protein (TBP) to promoters.
Title: Dissection of coactivator requirement at RNR3 reveals unexpected contributions from TFIID and SAGA.
Recruitment of Rap1 to the RNR3 gene is dependent on activation of the DNA damage checkpoint and chromatin remodelling by SWI/SNF.
Title: Yeast Rap1 contributes to genomic integrity by activating DNA damage repair genes.
Two histone deacetylases, RPD3 and HOS2, are required for the activation of DNA damage-inducible genes RNR3 and HUG1.
Title: Histone deacetylases RPD3 and HOS2 regulate the transcriptional activation of DNA damage-inducible genes.
nucleosome placement plays a dominant role in repression and that the ability of the core promoter to position a nucleosome is a major determinant in TAF(II) dependency of genes in vivo
Title: Exposing the core promoter is sufficient to activate transcription and alter coactivator requirement at RNR3.
The WTM genes amplify expression of the stress-inducible gene RNR3.
Title: The WTM genes in budding yeast amplify expression of the stress-inducible gene RNR3.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
contributes_to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IDA Inferred from Direct Assay more info	PubMed
enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in deoxyribonucleotide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in deoxyribonucleotide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in deoxyribonucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
part_of ribonucleoside-diphosphate reductase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonucleoside-diphosphate reductase complex	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: ribonucleotide-diphosphate reductase subunit RNR3

NP_012198.3

EC 1.17.4.1

Minor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

238099..240708 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179416.3 → NP_012198.3 TPA: ribonucleotide-diphosphate reductase subunit RNR3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012198.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVL8, P21672

UniProtKB/TrEMBL

A6ZVK7, B3LTR9, C7GVZ0

Conserved Domains (1) summary

PLN02437
Location:1 → 775

PLN02437; ribonucleoside--diphosphate reductase large subunit