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    GP second secreted glycoprotein;small secreted glycoprotein;spike glycoprotein [ Zaire ebolavirus ]

    Gene ID: 911829, updated on 6-Oct-2023

    Summary

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    Gene symbol
    GP
    Gene description
    second secreted glycoprotein;small secreted glycoprotein;spike glycoprotein
    Locus tag
    ZEBOVgp4
    Gene type
    protein coding
    RNA name
    sGP
    RefSeq status
    PROVISIONAL
    Organism
    Zaire ebolavirus (isolate: Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga)
    Lineage
    Viruses; ssRNA viruses; ssRNA negative-strand viruses; Mononegavirales; Filoviridae; Ebolavirus
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    Genomic context

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    Sequence:
    NC_002549.1 (5900..8305)

    NC_002549.1Genomic Context describing neighboring genes Neighboring gene nucleoprotein Neighboring gene polymerase complex protein Neighboring gene matrix protein Neighboring gene minor nucleoprotein Neighboring gene membrane-associated protein Neighboring gene RNA-dependent RNA polymerase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002549.1

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Recovery of infectious Ebola virus from complementary DNA: RNA editing of the GP gene and viral cytotoxicity. Volchkov VE, et al. Science, 2001 Mar 9. PMID 11239157
    2. Ebola virus-like particle-induced activation of NF-kappaB and Erk signaling in human dendritic cells requires the glycoprotein mucin domain. Martinez O, et al. Virology, 2007 Aug 1. PMID 17434557, Free PMC Article
    3. Differential expression of the Ebola virus GP(1,2) protein and its fragments in E. coli. Das D, et al. Protein Expr Purif, 2007 Jul. PMID 17383893
    4. The nonstructural small glycoprotein sGP of Ebola virus is secreted as an antiparallel-orientated homodimer. Volchkova VA, et al. Virology, 1998 Oct 25. PMID 9792851
    5. [Molecular mechanisms of Ebola virus reproduction]. Subbotina EL, et al. Vopr Virusol, 2007 Jan-Feb. PMID 17338228

    See all (115) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A Glycoprotein Mutation That Emerged during the 2013-2016 Ebola Virus Epidemic Alters Proteolysis and Accelerates Membrane Fusion.
      Title: A Glycoprotein Mutation That Emerged during the 2013-2016 Ebola Virus Epidemic Alters Proteolysis and Accelerates Membrane Fusion.
    2. A Virion-Based Assay for Glycoprotein Thermostability Reveals Key Determinants of Filovirus Entry and Its Inhibition.
      Title: A Virion-Based Assay for Glycoprotein Thermostability Reveals Key Determinants of Filovirus Entry and Its Inhibition.
    3. The EBOV glycoprotein (GP1,2) is the sole viral envelope protein and a major component of immunogenicity; it is encoded by the GP gene along with two truncated versions: soluble GP (sGP) and small soluble GP (ssGP). sGP may subvert the host immune response by inducing antibodies against sGP rather than GP1,2.
      Title: The Roles of Ebola Virus Soluble Glycoprotein in Replication, Pathogenesis, and Countermeasure Development.
    4. Growth-Adaptive Mutations in the Ebola Virus Makona Glycoprotein Alter Different Steps in the Virus Entry Pathway.
      Title: Growth-Adaptive Mutations in the Ebola Virus Makona Glycoprotein Alter Different Steps in the Virus Entry Pathway.
    5. the structure of the membrane proximal external region (MPER) connected to the TM domain: i.e., the missing parts of the EBOV GP2 structure. The structure, solved by solution NMR and EPR spectroscopy in membrane-mimetic environments, consists of a helix-turn-helix architecture that is independent of pH.
      Title: Structure of the Ebola virus envelope protein MPER/TM domain and its interaction with the fusion loop explains their fusion activity.
    6. Ebola virus GP directly subverts the host's immune response by increasing the susceptibility of monocytes to Ebola virus infection.
      Title: Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection.
    7. report provides experimental evidence that the spontaneous mutation T544I is a tissue culture adaptation in certain cell lines and that it may be unique for the species Zaire ebolavirus; the mutation led to a marked increase in virus growth kinetics and conferred a robust growth advantage over wild-type rVSV-MAK-GP on Vero E6 cells
      Title: Spontaneous Mutation at Amino Acid 544 of the Ebola Virus Glycoprotein Potentiates Virus Entry and Selection in Tissue Culture.
    8. although Asn(563) and Asn(618) are not required for EBOV GP expression, they synergistically regulate its maturation, which determines its functionality.
      Title: Mechanistic understanding of N-glycosylation in Ebola virus glycoprotein maturation and function.
    9. Study determined the crystal structure of the primed GP (GPcl) of Ebola virus bound to domain C of NPC1 (NPC1-C); NPC1-C utilizes two protruding loops to engage a hydrophobic cavity on head of GPcl. Upon enzymatic cleavage and NPC1-C binding, conformational change in the GPcl further affects the state of the internal fusion loop, triggering membrane fusion.
      Title: Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1.
    10. Study presents the 3.6 A crystal structure of Marburg virus (strain Ravn) GP in complex with a cross-reactive antibody, MR78, from a human survivor, and a lower resolution structure of the antibody bound to Ebola virus(strain Zaire) GP.
      Title: Structural basis for Marburg virus neutralization by a cross-reactive human antibody.
    Submit: New GeneRIF Correction See all GeneRIFs (30)

    General gene information

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    Homology

    regulatory

    • Loc: 5900-5911 regulatory_class = other
    • Loc: 6918-6924 function = RNA editing , regulatory_class = other
    • Loc: 8288-8299 regulatory_class = other
    • Loc: 8295-8305 regulatory_class = polyA_signal_sequence

    General protein information

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    Preferred Names
    second secreted glycoprotein;small secreted glycoprotein;spike glycoprotein
    NP_066246.1
    • virion spike glycoprotein precursor; an additional A residue is inserted during transcription; encodes two disulfide linked subunits GP1 and GP2
    NP_066247.1
    • sGP, small non-structural, secreted glycoprotein; sGP secreted as an anti-parallel oriented homodimer
    NP_066248.1
    • second non-structural secreted glycoprotein; super small secreted glycoprotein; secreted in a monomeric form; one A residue is deleted or two additional A residues are inserted at the editing site during transcription of the GP gene

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002549.1 Reference assembly

      Range
      5900..8305
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_066246.1 spike glycoprotein [Zaire ebolavirus]

      See identical proteins and their annotated locations for NP_066246.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      Q05320, Q66818, Q77LU5, Q8B9S1, Q8JS62
      UniProtKB/TrEMBL
      A0A089X075, A0A0E3NBB1, A9QPL9, L7QHR7
      Conserved Domains (3) summary
      cd09850
      Location:557 → 629
      Ebola-like_HR1-HR2; heptad repeat 1-heptad repeat 2 region of the transmembrane subunit of Filoviridae viruses, Ebola virus and Marburg virus, and related domains
      pfam01611
      Location:15 → 348
      Filo_glycop; Filovirus glycoprotein
      cl28464
      Location:341 → 493
      Spr; Cell wall-associated hydrolase, NlpC family [Cell wall/membrane/envelope biogenesis]

    2. NP_066247.1 small secreted glycoprotein [Zaire ebolavirus]

      See identical proteins and their annotated locations for NP_066247.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      O11458, O12421, O12717, P60170, P60171, P87670, Q66801, Q66819, Q6V1Q6, Q77LU4, Q8JS61, Q9YMG3
      UniProtKB/TrEMBL
      A0A089YMW7, A0A096YGX7, A0A0E3H7K0, A0A0G2YKH9, A0A2U9QPY9, A0A3G1S941, A9QPM0, I0DHB0, L7QI98
      Conserved Domains (1) summary
      pfam01611
      Location:15 → 298
      Filo_glycop; Filovirus glycoprotein

    3. NP_066248.1 second secreted glycoprotein [Zaire ebolavirus]

      See identical proteins and their annotated locations for NP_066248.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A096YGX1, A0A0G2YD57, A0A2U9QPX5, A0A3G1TWX0, A0A3G2LME3, G8DB42, L7QHT8, L7QHW1, Q6V1Q5
      Conserved Domains (1) summary
      pfam01611
      Location:15 → 295
      Filo_glycop; Filovirus glycoprotein
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    AAD14584.1
    AAD14585.1
    AAD14586.1

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