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    mhpC 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944954, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mhpC
    Official Full Name
    2-hydroxy-6-ketonona-2,4-dienedioate hydrolase
    Primary source
    ECOCYC:M012
    Locus tag
    b0349
    See related
    ASAP:ABE-0001201
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0346
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    Genomic context

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    Sequence:
    NC_000913.3 (371239..372105)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-(3-hydroxyphenyl)propanoate hydroxylase Neighboring gene 3-carboxyethylcatechol 2,3-dioxygenase Neighboring gene 2-hydroxypentadienoate hydratase Neighboring gene acetaldehyde dehydrogenase (acetylating) MhpF Neighboring gene 4-hydroxy-2-oxovalerate aldolase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Evidence for a gem-diol reaction intermediate in bacterial C-C hydrolase enzymes BphD and MhpC from 13C NMR spectroscopy. Li JJ, et al. Biochemistry, 2006 Oct 17. PMID 17029401
    2. Purification, characterization, and stereochemical analysis of a C-C hydrolase: 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase. Lam WW, et al. Biochemistry, 1997 Oct 7. PMID 9315862
    3. Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli. Bugg TD. Biochim Biophys Acta, 1993 Oct 6. PMID 8399388
    4. Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli. Burlingame R, et al. J Bacteriol, 1983 Jul. PMID 6345502, Free PMC Article
    5. Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation. Burlingame RP, et al. J Bacteriol, 1986 Oct. PMID 3531186, Free PMC Article

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. X ray structure
      Title: The structure of the C-C bond hydrolase MhpC provides insights into its catalytic mechanism.
    2. A catalytic mechanism is proposed involving stabilisation of reactive intermediates and activation of a nucleophilic water molecule by Ser110
      Title: Catalytic mechanism of C-C hydrolase MhpC from Escherichia coli: kinetic analysis of His263 and Ser110 site-directed mutants.
    3. Replacement of Arg-188 in MhpC with Gln and Lys led to 200- and 40-fold decreases, respectively, in k(cat).
      Title: Catalytic role for arginine 188 in the C-C hydrolase catalytic mechanism for Escherichia coli MhpC and Burkholderia xenovorans LB400 BphD.
    4. A new line-broadened signal was observed upon incubation of the (13)C-labeled substrate with an H114A MhpC mutant, which is able to accept the 6-phenyl-containing substrate, on a shorter time scale.
      Title: Evidence for a gem-diol reaction intermediate in bacterial C-C hydrolase enzymes BphD and MhpC from 13C NMR spectroscopy.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within 3-(3-hydroxy)phenylpropionate catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in 3-phenylpropionate catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    2-hydroxy-6-ketonona-2,4-dienedioate hydrolase
    NP_414883.5

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      371239..372105
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414883.5 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414883.5

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1NRN7
      Conserved Domains (1) summary
      TIGR03343
      Location:7 → 286
      biphenyl_bphD; 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

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