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    seqA negative modulator of initiation of replication [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945272, updated on 17-Dec-2024

    Summary

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    Official Symbol
    seqA
    Official Full Name
    negative modulator of initiation of replication
    Primary source
    ECOCYC:EG12197
    Locus tag
    b0687
    See related
    ASAP:ABE-0002343
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0675; hsm-1
    Summary
    SeqA is required for the membrane binding of hemimethylated oriC in vitro. [More information is available at EcoGene: EG12197]. The SeqA protein is a negative modulator of the initiation of chromosome replication and is required for sequestration of oriC, ensuring a single round of chromosome replication per cell cycle . [More information is available at EcoCyc: EG12197].
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    Genomic context

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    Sequence:
    NC_000913.3 (712987..713532)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ribbon-helix-helix domain-containing protein YbfE Neighboring gene esterase Neighboring gene phosphoglucomutase Neighboring gene lipoprotein YbfP

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Binding of SeqA protein to hemi-methylated GATC sequences enhances their interaction and aggregation properties. Han JS, et al. J Biol Chem, 2004 Jul 16. PMID 15151991
    2. SeqA protein aggregation is necessary for SeqA function. Lee H, et al. J Biol Chem, 2001 Sep 14. PMID 11457824
    3. Insights into negative modulation of E. coli replication initiation from the structure of SeqA-hemimethylated DNA complex. Guarné A, et al. Nat Struct Biol, 2002 Nov. PMID 12379844
    4. Identification of functional domains of the Escherichia coli SeqA protein. Fujikawa N, et al. Biochem Biophys Res Commun, 2003 Jan 17. PMID 12507506
    5. Excess SeqA prolongs sequestration of oriC and delays nucleoid segregation and cell division. Bach T, et al. EMBO J, 2003 Jan 15. PMID 12514137, Free PMC Article

    See all (105) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli.
      Title: Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli.
    2. Data indicate that the SeqA protein is localized at midcell for most of the D-period, but colocalizes with the origin region before cell division and initiation of replication.
      Title: Dynamic Escherichia coli SeqA complexes organize the newly replicated DNA at a considerable distance from the replisome.
    3. Data suggest that, in the absence of SeqA, the sister-chromatid cohesion 'safety spacer' is destabilized and completely disappears if the replication fork is inhibited.
      Title: Replication fork inhibition in seqA mutants of Escherichia coli triggers replication fork breakage.
    4. We propose that SeqA binding results in loose inter-duplex junctions that are resistant to Topo IV cleavage
      Title: Regulation of sister chromosome cohesion by the replication fork tracking protein SeqA.
    5. Cell cycle analysis showed that loss of SeqA from the old replication forks did not occur at initiation of the new forks, but instead occurs at a time point coinciding with the end of SeqA-dependent origin sequestration.
      Title: Replication fork movement and methylation govern SeqA binding to the Escherichia coli chromosome.
    6. The number of SeqA foci is not directly governed by the number of replication forks, and supports the idea that new DNA may be 'captured' by existing SeqA structures.
      Title: Growth rate dependent numbers of SeqA structures organize the multiple replication forks in rapidly growing Escherichia coli.
    7. well-defined SeqA structures are not found in situations of extensive replication fork repair
      Title: A reduction in ribonucleotide reductase activity slows down the chromosome replication fork but does not change its localization.
    8. An examination of the molecular model of Escherichia coli SeqA protein, a negative regulator of chromosome DNA replication which prevents the overinitiation of replication.
      Title: Modeling of the full-length Escherichia coli SeqA protein, in complex with DNA.
    9. seqA mutants have increased LPS phosphorylation and exhibit high levels of chromosomal fragmentation.
      Title: Reduced lipopolysaccharide phosphorylation in Escherichia coli lowers the elevated ori/ter ratio in seqA mutants.
    10. These results indicate that unlike oriC, SeqA modulates lambda DNA replication indirectly, most probably by influencing the stability of the lambda replication complex and the transcriptional activation of ori lambda.
      Title: Modulation of lambda plasmid and phage DNA replication by Escherichia coli SeqA protein.
    Submit: New GeneRIF Correction See all GeneRIFs (18)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA replication origin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables double-stranded methylated DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hemi-methylated DNA-binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sequence-specific DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in negative regulation of DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of DNA-templated DNA replication initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within negative regulation of DNA-templated DNA replication initiation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of DNA-templated DNA replication initiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within nucleoid organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within sister chromatid cohesion IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of SeqA-DNA complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    negative modulator of initiation of replication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      712987..713532
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415213.1 negative modulator of initiation of replication [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415213.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      D3GXD9
      Conserved Domains (1) summary
      PRK11187
      Location:1 → 181
      PRK11187; replication initiation negative regulator SeqA
    Nucleotide Protein
    Heading Accession and Version

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