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    cas3 CRISPR-associated endonuclease/helicase Cas3 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947229, updated on 3-Dec-2024

    Summary

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    Official Symbol
    cas3
    Official Full Name
    CRISPR-associated endonuclease/helicase Cas3
    Primary source
    ECOCYC:EG12634
    Locus tag
    b2761
    See related
    ASAP:ABE-0009054
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2756; ygcB
    Summary
    The Cascade antiviral complex is comprised of CasABCDE and short CRISP RNAs (crRNAs) and requires the predicted helicase YgcB(Cas3) for activity (Brouns , 2008). [More information is available at EcoGene: EG12634]. In the presence of Cas3, the Cascade complex gives rise to resistance against phages whose genomes have regions of complementarity to elements in the CRISPR repeat . [More information is available at EcoCyc: EG12634].
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    Genomic context

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    Sequence:
    NC_000913.3 (2884553..2887219, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene type I-E CRISPR system Cascade subunit CasC Neighboring gene type I-E CRISPR system Cascade subunit CasB Neighboring gene type I-E CRISPR system Cascade subunit CasA Neighboring gene ncRNA Neighboring gene phosphoadenosine phosphosulfate reductase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A Tryptophan 'Gate' in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity. He L, et al. Int J Mol Sci, 2021 Mar 11. PMID 33799639, Free PMC Article
    2. Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3. Yoshimi K, et al. Nat Commun, 2022 Aug 30. PMID 36042215, Free PMC Article
    3. Cas3-stimulated runaway replication of modified ColE1 plasmids in Escherichia coli is temperature dependent. Radovčić M, et al. FEMS Microbiol Lett, 2019 May 1. PMID 31095294
    4. Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation. Künne T, et al. Mol Cell, 2016 Sep 1. PMID 27546790
    5. CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3. Westra ER, et al. Mol Cell, 2012 Jun 8. PMID 22521689, Free PMC Article

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3.
      Title: Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3.
    2. A Tryptophan 'Gate' in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity.
      Title: A Tryptophan 'Gate' in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity.
    3. Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage.
      Title: Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage.
    4. We found that Cas3-stimulated uncontrolled plasmid replication occurs only at 37 degrees C, irrespective of the genotype of the analysed mutants, and dependent on Cas3 helicase function.
      Title: Cas3-stimulated runaway replication of modified ColE1 plasmids in Escherichia coli is temperature dependent.
    5. Cas3-derived target DNA degradation fragments fuel primed CRISPR adaptation.
      Title: Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation.
    6. Transcription and stability of cas3 in Deltahns cells is limiting for resistance to phage.
      Title: Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS.
    7. These results establish Cas1-Cas2 as a trans-acting factor necessary for the recruitment and regulation of Cas3 at escape targets. Based on findings, a mechanistic framework is proposed describing how Cascade, Cas1, Cas2, and Cas3 work together to process and disable foreign genetic elements.
      Title: Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System.
    8. study observed that Cas3 promoted runaway ColE1 plasmid replication, requiring Cas3 helicase activity; Cas3 expression antagonized RNaseHI, observed as a concatamer phenotype, but also required RNaseHI for its ability to stimulate plasmid copy number
      Title: Cas3 stimulates runaway replication of a ColE1 plasmid in Escherichia coli and antagonises RNaseHI.
    9. Cas3 is involved in DNA degradation and invader neutralization.
      Title: CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3.
    10. Study discuss possible overlapping roles of Cas3 and Cascade in E. coli, and the apparently antagonistic roles of Cas3 catalysing RNA-DNA annealing and ATP-dependent helicase unwinding.
      Title: Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    General protein information

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    Preferred Names
    CRISPR-associated endonuclease/helicase Cas3

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2884553..2887219 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417241.1 CRISPR-associated endonuclease/helicase Cas3 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417241.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6N8R059
      Conserved Domains (1) summary
      PRK09694
      Location:1 → 883
      PRK09694; CRISPR-associated helicase/endonuclease Cas3
    Nucleotide Protein
    Heading Accession and Version

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