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    polA DNA polymerase I [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948356, updated on 3-Dec-2024

    Summary

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    Official Symbol
    polA
    Official Full Name
    DNA polymerase I
    Primary source
    ECOCYC:EG10746
    Locus tag
    b3863
    See related
    ASAP:ABE-0012618
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3855; resA
    Summary
    Residues 324-928 constitute the Klenow fragment. [More information is available at EcoGene: EG10746]. DNA Polymerase I (Pol I) is a multifunctional enzyme that combines a DNA polymerase activity, a 5' to 3' exonuclease activity and a 3' to 5' proofreading exonuclease activity. [More information is available at EcoCyc: EG10746].
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    Genomic context

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    Sequence:
    NC_000913.3 (4046966..4049752)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF945 domain-containing protein YihF Neighboring gene 1-acylglycerol-3-phosphate O-acyltransferase YihG Neighboring gene ncRNA Neighboring gene GTP-binding protein YihA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Thermodynamic dissection of the polymerizing and editing modes of a DNA polymerase. Bailey MF, et al. J Mol Biol, 2004 Feb 20. PMID 15095980
    2. Discrimination against purine-pyrimidine mispairs in the polymerase active site of DNA polymerase I: a structural explanation. Minnick DT, et al. Proc Natl Acad Sci U S A, 2002 Feb 5. PMID 11830658, Free PMC Article
    3. Participation of active-site carboxylates of Escherichia coli DNA polymerase I (Klenow fragment) in the formation of a prepolymerase ternary complex. Gangurde R, et al. Biochemistry, 2002 Dec 10. PMID 12463755
    4. Functional implications of the Klenow fragment structure. Steitz TA, et al. Biochem Soc Trans, 1986 Apr. PMID 3519313
    5. Interaction of DNA polymerase I (Klenow fragment) with the single-stranded template beyond the site of synthesis. Turner RM Jr, et al. Biochemistry, 2003 Mar 4. PMID 12600204

    See all (184) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Single-molecule view of coordination in a multi-functional DNA polymerase.
      Title: Single-molecule view of coordination in a multi-functional DNA polymerase.
    2. E. coli DNA Polymerase I and the Klenow Fragment.
      Title: E. coli DNA Polymerase I and the Klenow Fragment.
    3. Findings indicate an unrealized function of DNA polymerase I (Pol I), and a possible mechanism by which G4 structures can be resolved during DNA replication and/or repair.
      Title: Escherichia coli DNA polymerase I can disrupt G-quadruplex structures during DNA replication.
    4. Authors demonstrate for the first time that the major DNA polymerases (Pol I and Pol III) and DNA ligase are directly involved with oligo recombination.
      Title: Bacterial DNA polymerases participate in oligonucleotide recombination.
    5. Pol I catalyzes DNA synthesis past N6-dA-linked peptide cross-links and is likely to play an role in cellular bypass of large major groove DNA lesions.
      Title: Role of high-fidelity Escherichia coli DNA polymerase I in replication bypass of a deoxyadenosine DNA-peptide cross-link.
    6. analysis of complexes of DNA polymerase I (Klenow fragment) for base and sugar discrimination during nucleotide substrate selection
      Title: Distinct complexes of DNA polymerase I (Klenow fragment) for base and sugar discrimination during nucleotide substrate selection.
    7. Data show that dnaX36 strains have an increased requirement for DNA polymerase I.
      Title: Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant.
    8. Findings indicate that elevated levels of Pol I partially suppress the temperature-sensitive growth phenotype of the dnaN159 mutant strain.
      Title: Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.
    9. mechanism in which Arg668 forms a hydrogen bonding fork between the minor groove of the primer terminus and the ring oxygen of the deoxyribose moiety of the incoming dNTP to align the 3'-hydroxyl group with the alpha-phosphate of the dNTP
      Title: Fidelity of mispair formation and mispair extension is dependent on the interaction between the minor groove of the primer terminus and Arg668 of DNA polymerase I of Escherichia coli.
    10. mutant(Delta)polA is defective in base mismatch correction
      Title: Escherichia coli mutator (Delta)polA is defective in base mismatch correction: the nature of in vivo DNA replication errors.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-5' exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3'-5' exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 5'-3' exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 5'-3' exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables catalytic activity, acting on a nucleic acid IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA-templated DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA-templated DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within base-excision repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in double-strand break repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase I
    NP_418300.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4046966..4049752
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418300.1 DNA polymerase I [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418300.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A895NWI0
      Conserved Domains (1) summary
      PRK05755
      Location:6 → 928
      PRK05755; DNA polymerase I; Provisional
    Nucleotide Protein
    Heading Accession and Version

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