U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination
    • Showing Current items.

    tgt tRNA-guanine transglycosylase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949130, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    tgt
    Official Full Name
    tRNA-guanine transglycosylase
    Primary source
    ECOCYC:EG10996
    Locus tag
    b0406
    See related
    ASAP:ABE-0001413
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0400; vacC(S.f.)
    Summary
    Tgt forms a transient covalent linkage with the tRNA substrate. [More information is available at EcoGene: EG10996]. The tgt gene encodes tRNA-guanine transglycosylase . [More information is available at EcoCyc: EG10996].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (426137..427264)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene acyl carrier protein phosphodiesterase Neighboring gene tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase Neighboring gene Sec translocon accessory complex subunit YajC Neighboring gene Sec translocon accessory complex subunit SecD

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A versatile and general prokaryotic expression vector, pLACT7. Chong S, et al. Biotechniques, 1994 Oct. PMID 7833029
    2. tRNA-guanine transglycosylase from Escherichia coli: molecular mechanism and role of aspartate 89. Kittendorf JD, et al. Biochemistry, 2001 Nov 27. PMID 11714265
    3. tRNA recognition by tRNA-guanine transglycosylase from Escherichia coli: the role of U33 in U-G-U sequence recognition. Nonekowski ST, et al. RNA, 2001 Oct. PMID 11680848, Free PMC Article
    4. tRNA-guanine transglycosylase from Escherichia coli: recognition of noncognate-cognate chimeric tRNA and discovery of a novel recognition site within the TpsiC arm of tRNA(Phe). Kung FL, et al. RNA, 2000 Feb. PMID 10688362, Free PMC Article
    5. Cysteine 265 is in the active site of, but is not essential for catalysis by tRNA-guanine transglycosylase (TGT) from Escherichia coli. Garcia GA, et al. J Protein Chem, 1997 Jan. PMID 9055203

    See all (49) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. mutation of key residues in the E. coli TGT active site can ''open up'' the site to allow for the binding of competitive pteridine inhibitors.
      Title: Differential heterocyclic substrate recognition by, and pteridine inhibition of E. coli and human tRNA-guanine transglycosylases.
    2. phylogenetic and kinetic analyses support the conclusion that all tRNA-guanine transglycosylases have divergently evolved to specifically recognize their cognate heterocyclic substrates.
      Title: Evolution of eukaryal tRNA-guanine transglycosylase: insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases.
    3. N-terminus verified by Edman degradation on complete protein
      Title: tRNA-guanine transglycosylase from Escherichia coli. Overexpression, purification and quaternary structure.
    4. The key role of aspartate 143 in maintaining the anticodon identities of TGT queuine-containing tRNAs is confirmed.
      Title: Role of aspartate 143 in Escherichia coli tRNA-guanine transglycosylase: alteration of heterocyclic substrate specificity.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA-guanosine(34) queuine transglycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA-guanosine(34) queuine transglycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in queuosine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in queuosine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within queuosine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA wobble guanine modification IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within tRNA wobble guanine modification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA-guanine transglycosylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    tRNA-guanine transglycosylase
    NP_414940.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      426137..427264
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414940.1 tRNA-guanine transglycosylase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414940.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7A7FCP2
      Conserved Domains (1) summary
      PRK00112
      Location:1 → 364
      tgt; queuine tRNA-ribosyltransferase; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.