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ERX1806141: Illumina HiSeq 1000 paired end sequencing; RNA-seq analysis of date-palms treated with stress hormone ABA compared to controls
2 ILLUMINA (Illumina HiSeq 1000) runs: 24.7M spots, 4.9G bases, 2.7Gb downloads

Design: RNA-seq analysis of date-palms treated with stress hormone ABA compared to controls
Submitted by: CUBE Computation Systems Biology Vienna 1090 Austria (CUBE Computation Systems Biology Vienna 1090 Austr)
Study: RNA-seq analysis of date-palms treated with stress hormone ABA compared to controls
show Abstracthide Abstract
Date palms are extremely stress tolerant. The aim of the experiment was to evaluate, how date palm leaves react to application of the drought stress hormone ABA on the transcriptional level, and to compare the resulting data with those obtained from the stress sensitive Arabidopsis thaliana. The experiment thus should help us to understand how date palms manage their stress tolerance in comparison to sensitive plants. Single cut pinneates from date palm leaves were treated for 2 h with CO2-free air in darkness, then illuminated for 4 h at a photon flux rate of 620 µmol m2 s-1. Following this pre-incubation, 25 µM ABA was fed via the petiole. After 2 h, RNA was extracted from the pinneates. Controls were treated equally with ABA-free solution.
Sample: WU19
SAMEA4553950 • ERS1453129 • All experiments • All runs
Library:
Name: WU19_p
Instrument: Illumina HiSeq 1000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: 2h ABA teated and control pinneates from data palm leaves were used. ABA was fed through the petiole and was applied with a final concentration of 25µM in the leaves’ water reservoir as described (Bemm et al. (2016). Genome Res. 26(6):812-825) NEBNext Ultra™ RNA Library Prep protocol (New England Biolabs, Ipswich, MA, USA)
Spot descriptor:
forward101  reverse

Experiment attributes:
Experimental Factor: abscisic acid: compound
Runs: 2 runs, 24.7M spots, 4.9G bases, 2.7Gb
Run# of Spots# of BasesSizePublished
ERR173637712,355,6062.5G1.4Gb2017-08-02
ERR173637812,322,1452.5G1.4Gb2017-08-02

ID:
4336065

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