Staphylococcus pseudintermedius strain 157588 - SRA

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SRX8397207: Staphylococcus pseudintermedius strain 157588
1 ILLUMINA (Illumina Genome Analyzer) run: 11.5M spots, 3.4G bases, 646.1Mb downloads

Design: The paired-end sequencing library was prepared using NEBNext Ultra DNA Library Prep Kit for Illumina. The library preparation process was initiated with 200ng DNA. The DNA sample was mechanically sheared into smaller fragments by Covaris followed by continuous step of end-repair where an A is added to the 3 ends making the DNA fragments ready for adapter ligation. Following this step, platform-specific adapters are ligated to both ends of the DNA fragments. These adapters contain sequences essential for binding dual-barcoded library to a flow cell for sequencing, allowing for PCR amplification of adapter-ligated fragments, and binding standard Illumina sequencing primers. To ensure maximum yields from limited amounts of starting material, a high-fidelity amplification step was performed using HiFi PCR Master Mix.

Submitted by: Tamil Nadu Veterinary and Animal Sciences University

Study: Staphylococcus pseudintermedius strain:157588 Genome sequencing and assembly

PRJNA634609 • SRP264659 • All experiments • All runs

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To study the epidemiological and geographical differences in Staphylococcus pseudintermedius strain 157588 by whole genome sequencing

Sample: Staphylococcus pseudintermedius strain 157588

SAMN15002157 • SRS6710043 • All experiments • All runs

Organism: Staphylococcus pseudintermedius

Library:

Name: SP-1

Instrument: Illumina Genome Analyzer

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 11.5M spots, 3.4G bases, 646.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR11846863	11,523,171	3.4G	646.1Mb	2020-05-26

ID:: 10916247

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