WGS of Bos Taurus: Nasal swab extract - SRA

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SRX10130430: WGS of Bos Taurus: Nasal swab extract
1 ILLUMINA (Illumina NovaSeq 6000) run: 28.7M spots, 4.3G bases, 1.3Gb downloads

Design: Isolated genomic DNA was quantified with Qubit 2.0 DNA HS Assay (ThermoFisher, MA, USA) and quality assessed by Tapestation genomic DNA Assay (Agilent Technologies, CA, USA). Library preparation was performed using NexteraXT library kit (Illuma, CA, USA) per manufacturers recommendations. Final library quantity was measured by KAPA SYBR FAST qPCR with QuantStudio 5 System (Applied Biosystems, CA, USA), and library quality was evaluated by TapeStation HSD1000 ScreenTape (Agilent Technologies, CA, USA). Illumina 8-nt dual-indices were used. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina NovaSeq (Illumina, CA, USA) with a read length configuration of 150 (paired-end) PE for 40 M PE reads (20M in each direction) per sample.

Submitted by: Zagazig University

Study: Metagenomic analysis of nasal virome of Egyptian cattle with respiratory diseases

PRJNA702539 • SRP307086 • All experiments • All runs

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We aim here to characterize nasal virome of Egyptian cattle with clinical respiratory signs. The goal of this study is to discover viruses, particularly DNA viruses that are associated with respiratory disease complex (BRD) in cattle.

Sample:

SAMN17974853 • SRS8284586 • All experiments • All runs

Organism: bovine metagenome

Library:

Name: 20133XD-01-01

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: METAGENOMIC

Selection: Oligo-dT

Layout: PAIRED

Runs: 1 run, 28.7M spots, 4.3G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR13743319	28,655,927	4.3G	1.3Gb	2021-02-19

ID:: 13258006

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