Evolutionary network genomics of wood formation in a phylogenetic sur... - SRA

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SRX6604342: Evolutionary network genomics of wood formation in a phylogenetic survey of angiosperm forest trees
1 ILLUMINA (Illumina HiSeq 4000) run: 27.3M spots, 1.4G bases, 470.8Mb downloads

Design: Woody tissues were harvested from the middle third of the plant by lightly scrapping the xylem side of a debarked stem using a double-edged razor blade. For gravi-stimulated plants, developing xylem was separately harvested from the upper surface (tension wood) and from the lower surface (opposite wood) of a leaning stem. For time zero samples, two upright grown trees were randomly divided in half and independent xylem samples wereharvested. All tissue was immediately flash frozen in liquid nitrogen and stored at -80C. Frozen tissue was ground to a fine powder in liquid nitrogen and total RNA was extracted using TRIzol reagent. RNA samples were subsequently cleaned using a Qiagen RNeasy on-column DNase treatment. RNA sequencing libraries were generated using KAPA mRNA Hyper Prep Kit and multiplexed using 12 unique adapter sequences . Multiplexed samples were sequenced using 50 bp single-end runs on an Illumina HiSeq 4000 system.

Submitted by: Forest Service

Study: Evolutionary network genomics of wood formation in a phylogenetic survey of angiosperm forest trees

PRJNA556244 • SRP216575 • All experiments • All runs

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In this project, we modeled changes in gene co-expression relationships associated with the evolution of wood formation in a phylogenetic survey of 13 angiosperm tree species from the genera Liriodendron, Liquidambar, Eucalyptus, Populus and Salix.

Sample: AGSZ004_1_S49_L005_R1_001

SAMN12344436 • SRS5169319 • All experiments • All runs

Organism: Populus trichocarpa x Populus deltoides

Library:

Name: AGSZ004_1_S49_L005_R1_001

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 27.3M spots, 1.4G bases, 470.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR9849644	27,349,886	1.4G	470.8Mb	2020-08-01

ID:: 8710925

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