33 - SRA - NCBI

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SRX7502011: 33
1 ILLUMINA (Illumina HiSeq 2500) run: 6.6M spots, 2G bases, 761Mb downloads

Design: Qualified DNA is cut into fragments by restriction enzyme. The construction of the DNA libraries is through the processes of end repairing, adding A to tails, purification, PCR amplification and etc. Libraries are sequenced by Illumina high-throughput sequencer with paired-end sequencing strategy.

Submitted by: Khon Kaen University

Study: Whole-genome sequence analysis and comparisons between genotypic heteroresistance and minimum inhibitory concentrations of Mycobacterium tuberculosis isolates causing M/XDR-TB

PRJNA598981 • SRP239473 • All experiments • All runs

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Drug resistance remains a major challenge for tuberculosis control. Whole-genome sequencing (WGS) provides the highest genetic resolution for drug susceptibility tests (DST). We compared drug-susceptibility profiles of 62 M. tuberculosis isolates based on agar proportion, minimum inhibitory concentration (MIC) test and a WGS-based DST. The association between drug-resistance mutations and genotypic heteroresistance detected by WGS analysis and MIC of drugs was investigated.

Sample: 33

SAMN13735693 • SRS5943410 • All experiments • All runs

Organism: Mycobacterium tuberculosis

Library:

Name: M-37032

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: GENOMIC

Selection: Restriction Digest

Layout: PAIRED

Runs: 1 run, 6.6M spots, 2G bases, 761Mb

Run	# of Spots	# of Bases	Size	Published
SRR10828621	6,552,979	2G	761Mb	2020-01-05

ID:: 9798387

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