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SRX8480887: GSM4593193: WT; Deinococcus geothermalis DSM 11300; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 16.4M spots, 3.3G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptomic analysis of a putative LysR family regulator, a putative Dps, and a cystine importer disrupted mutants in radiation resistant bacterium Deinococcus geothermalis
show Abstracthide Abstract
Here, we compared gene expression level by RNA-Seq analysis among a wild-type (WT) Deinococcus geothermalis strain, a dgeo_2840 of LysR family member gene (?dgeo_2840), a putative Dps dgeo_0257 (?dgeo_0257), and a cystine importer dgeo_1986-1987 gene disrupted strain (?dgeo_1986-1987). From this transcriptomic data, we detected dgeo_2840, dgeo_0257, and dgeo_1986-1987 controlling genes which were somehow up-regulated and/or down-regulated. In particular, Deinococcus geothermalis has a total of 73 insertion sequences (ISs) in genomes, and some of them are actively transposed to other loci with replicative mode by the oxidative stress of hydrogen peroxide treatment. Overall design: 4 samples including WT and 3 mutant strains.
Sample: WT
SAMN15147265 • SRS6780787 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: The total RNA was extracted by the RIDOEx reagent (GeneAll, Korea). The extracted total RNA was purified using an RNeasy Mini Purification Kit (Qiagen, Germany) and RNase-Free DNase I Set (Qiagen, Germany). We commissioned D. geothermalis bacterial RNA-Seq, and data analysis was performed using the ExDEGA analysis tool of e-biogen Co. (Seoul, Korea).
Experiment attributes:
GEO Accession: GSM4593193
Links:
Runs: 1 run, 16.4M spots, 3.3G bases, 1.6Gb
Run# of Spots# of BasesSizePublished
SRR1193555416,365,5653.3G1.6Gb2020-06-07

ID:
11022382

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