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SRX8839033: WGS of Pectobacterium brasiliense: strain NAK:470
1 ILLUMINA (Illumina HiSeq 2500) run: 4.2M spots, 1.2G bases, 396.8Mb downloads

Design: Libraries for Illumina sequencing were constructed on randomly sheared DNA fragments (Covaris E210) aiming 300 to 500 bp insert size using the NEXTflex ChIP-seq Library prep kit (BIOO Scientific). Library sizes were analyzed on a Bioanalyzer (Agilent) and quantified using Qubit (Life Technologies) equimolar pooled. The library pool was loaded on an Illumina paired-end flowcell for cluster generation using a cBot (illumina). Sequencing was done on an Illumina HiSeq2500 instrument using 125, 7, 125 flow cycles for forward, index and reverse reads respectively
Submitted by: Wageningen Plant Research
Study: Pectobacterium genome sequencing for pangenomics
show Abstracthide Abstract
Expand the set of available Pectobacterium genomes and the representation of agriculturally relevant strains. The genomes are used for a pangenomic investigation of the genus.
Sample:
SAMN15660288 • SRS7102329 • All experiments • All runs
Library:
Name: BPHSRR0000069
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 4.2M spots, 1.2G bases, 396.8Mb
Run# of Spots# of BasesSizePublished
SRR123392184,151,3321.2G396.8Mb2021-02-02

ID:
11495399

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