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SRX8979431: Metatranscriptome of Wasmannia auropunctata: Adult worker
1 ILLUMINA (Illumina NovaSeq 6000) run: 24.1M spots, 7.2G bases, 2.1Gb downloads

Design: Total RNA was obtained from Wasmannia auropunctata ants by the Trizol and PureLink methods. mRNA from was enriched using oligo(dT) beads and rRNA removed using the Ribo-Zero kit. Library construction was completed by randomly fragmenting the mRNA. cDNA was synthesized using mRNA template and random hexamers primers, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. Sequence adapters were ligated to the cDNA, the double-stranded cDNA library was completed by size selection and PCR enrichment. The qualified libraries were sequenced on an Illumina (Miseq chemistry) sequencer after pooling according to its effective concentration and expected data volume.
Submitted by: United States Department of Agriculture, Agricultural Research Service
Study: Wasmannia auropunctata Metagenome
PRJNA658153 • SRP278269 • All experiments • All runs
show Abstracthide Abstract
The identification of RNA viruses in the invasive Little fire ant Wasmannia auropunctata.
Sample: Arg2 metatranscriptome from host Wasmannia auropunctata workers
SAMN15863223 • SRS7234454 • All experiments • All runs
Organism: ant metagenome
Library:
Name: Arg2
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: METATRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Runs: 1 run, 24.1M spots, 7.2G bases, 2.1Gb
Run# of Spots# of BasesSizePublished
SRR1248762824,061,1607.2G2.1Gb2024-08-19

ID:
11673665

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