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SRX9023318: GSM4749565: KPC PDA tumour 6 CD105pos CAFs; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 30.9M spots, 4.7G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)
Study: Gene expression profiles of CD105pos and CD105neg cancer-associated fibroblasts (CAFs) from KPC mice
PRJNA659726 • SRP279042 • All experiments • All runs
show Abstracthide Abstract
CD105pos and CD105neg CAFs were isolated from KPC pancreatic ductal adenocarcinoma tumours and gene expression profiles measured Overall design: Single live LINnegPDPNposCD90posCD105pos and CD105neg CAFs were FACS isolated from n=6 different KPC pancreatic ductal adenocarcinoma tumours, RNA isolated and gene expresion measured by next gen sequencing using standard methods
Sample: KPC PDA tumour 6 CD105pos CAFs
SAMN15923922 • SRS7274367 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: FACS isolated CAFs were centrifuged at 400g for 5 min, carefully aspirated and cells lysed in RLT buffer. Lysis and subsequent RNA isolation was conudcted as per instructions ffor the Qiagen RNeasy micro kits RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM4749565
Links:
Runs: 1 run, 30.9M spots, 4.7G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR1253341730,862,1374.7G1.7Gb2020-08-28

ID:
11728359

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