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SRX10404580: GSM5194624: Gut_Clean_GFP_rep1; Ixodes scapularis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 73.4M spots, 14.8G bases, 4.2Gb downloads

Submitted by: NCBI (GEO)
Study: Tick Adiponectin Receptor-Mediated Phospholipid Metabolism Contributes to Borrelia burgdorferi Colonization
show Abstracthide Abstract
Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin, suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection, suggesting that ISARL signaling may be co-opted by the Lyme disease agent. Consistent with this, RNA interference (RNAi)-mediated silencing of ISARL significantly reduced the B. burgdorferi burden in the tick. RNA-seq-based transcriptomics and RNAi assays demonstrate that ISARL-mediated phospholipid metabolism by phosphatidylserine synthase I is associated with B. burgdorferi survival. Furthermore, the tick complement C1q-like protein 3 interacts with ISARL, and B. burgdorferi facilitates this process. This study identifies a new tick metabolic pathway that is connected to the life cycle of the Lyme disease spirochete. Overall design: (1) Tick gut global gene expression change after silencing adiponectin receptor Examination of tick gut response by silencing adiponectin receptor after engorgement on Borrelia burgdorferi-infected or uninfected mice, using RNA-seq (2) Tick gut global gene expression change after injecting recombinant GFP and adiponectin protein Examination of tick gut response after injecting recombinant GFP and adiponectin protein, using RNA-seq
Sample: Gut_Clean_GFP_rep1
SAMN18397200 • SRS8527049 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Tick gut was dissected, and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5194624
Links:
Runs: 1 run, 73.4M spots, 14.8G bases, 4.2Gb
Run# of Spots# of BasesSizePublished
SRR1402797973,364,86614.8G4.2Gb2021-12-09

ID:
13722731

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