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SRX10499329: GBS of Pisum Sativum
1 ILLUMINA (HiSeq X Ten) run: 6.3M spots, 1.7G bases, 623.8Mb downloads

Design: DNA from CREA germplasm collection was extracted from leaf green tissues using the DNeasy Plant Mini Kit (Qiagen) and checked for integrity on 1% agarose gel. DNA quantitation was performed using the Quant-iTTM PicoGreen dsDNA assay kit (Life Technologies). A GBS library was prepared using the ApeKI restriction enzyme, following the protocol described by Elshire et al. Library sequencing was performed using the Illumina HiSeq X platform and paired-end runs (2 x 150 bp).
Submitted by: CREA - Council for Agricultural Research and Analysis of Agricultural Economics
Study: GBS sequencing of CREA pea world collection
show Abstracthide Abstract
GBS sequencing of a pea (pisum sativum) world collection owned by CREA-ZA (Italy), comprising 234 accessions.
Sample: B90
SAMN18586246 • SRS8623988 • All experiments • All runs
Organism: Pisum sativum
Library:
Name: plate1_2G1
Instrument: HiSeq X Ten
Strategy: RAD-Seq
Source: GENOMIC
Selection: Restriction Digest
Layout: PAIRED
Runs: 1 run, 6.3M spots, 1.7G bases, 623.8Mb
Run# of Spots# of BasesSizePublished
SRR141290316,319,6011.7G623.8Mb2025-01-01

ID:
13933102

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