Spinach leaf bacterial microbiome - SRA

Links from BioSample

SRX10525456: Spinach leaf bacterial microbiome
1 ILLUMINA (NextSeq 500) run: 33.1M spots, 9.8G bases, 4Gb downloads

Design: 25 grams spinach were mixed with 225 ml diluent (0.1% peptone water and 0.1% Tween 80) in a stomacher bag. (...) The suspensions were passed through a 40 m membrane filter to remove large leave debris, followed by membrane filtration using a 0.22 m filter to collect the bacterial cells. The membrane carrying bacterial cells was removed from the filtration unit and cut into small pieces for DNA isolation. Metagenomic DNA was extracted using the Zymobiomics kit following the manufacturers instructions. Sequencing libraries were prepared using NEBNext Ultra II DNA Prep kit for Illumina. DNA sequencing was performed in Illumina NextSeq 500 instrument using mid output kit (2 150bp) at the Genomics Core Laboratory at UAMS

Submitted by: University of Arkansas for Medical Sciences

Study: Assessment of Antibiotic Resistance in Vegetables in the United States

PRJNA705736 • SRP308931 • All experiments • All runs

show Abstracthide Abstract

Bacterial strains resistant to beta-lactam antibiotics were isolated from vegetables in the United States. Shotgun metagenomic sequencing of microbiome from vegetables was performed.

Sample: spinach leaf microbiome

SAMN18640441 • SRS8648970 • All experiments • All runs

Organism: leaf metagenome

Library:

Name: Spinach_1

Instrument: NextSeq 500

Strategy: OTHER

Source: METAGENOMIC

Selection: unspecified

Layout: PAIRED

Runs: 1 run, 33.1M spots, 9.8G bases, 4Gb

Run	# of Spots	# of Bases	Size	Published
SRR14156940	33,126,502	9.8G	4Gb	2021-04-07

ID:: 13975578

SRA

Sequence Read Archive

Result Filters

Send to:

Links from BioSample

Supplemental Content

Related information

Recent activity