show Abstracthide AbstractThis study includes genomes from 194 Bacteroides fragilis group isolates, including 174 isolates collected at the Barnes-Jewish Hospital in St. Louis, MO from 2018-2020, as well as 20 banked B. fragilis sensu stricto isolates enriched for carbapenem resistance. Genome sequences are used for phylogenomic studies to validate the accuracy of species identification tools commonly used in clinical settings. Furthermore, these genomes are used to identify species-specific genetic elements related to antibiotic resistance for comparison with phenotypic antibiotic resistance.