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SRX13249494: WGS Illumina for Serratia marcescens: Felis catus urine
1 ILLUMINA (Illumina HiSeq 2500) run: 452,075 spots, 271.2M bases, 162.5Mb downloads

Design: Genomic DNA extracted with Promega Wizard Genomic DNA Purification Kit and stored at -20C. Extracted DNA quantified using a Quantus Fluorometer with QuantiFluor One dsDNA reagant. RNA/DNA ratios checked using the Nanodrop (v3.8.1) spectrophotometer (Thermo Fisher Scientific). Genomic DNA quality testing, library preparation using the Nextera XT DNA library kit v3.0 and Illumina short read sequencing of the extracted DNA conducted in accordance with the manufacturers instructions by the Australian Genome Research Facility. Reads filtered for quality scores >20 and adapters removed using the wrapper Trim Galore v0.4.4.
Submitted by: The University of Melbourne
Study: Nosocomial infections in a veterinary hospital
show Abstracthide Abstract
We examined a collection of Serratia spp. isolated from diverse patients at a large veterinary teaching hospital in Melbourne, Australia. We assessed their taxonomic identification, genomic diversity, and presence of antimicrobial resistance genes. We focused on a group of quasi-clonal strains isolated from complicated surgical procedures, which were found to be chlorhexidine tolerant and carried a IncHI2 plasmid containing multiple drug resistances.
Sample: Microbe sample from Serratia marcescens
SAMN23485797 • SRS11172846 • All experiments • All runs
Library:
Name: CM2012_118_i
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 452,075 spots, 271.2M bases, 162.5Mb
Run# of Spots# of BasesSizePublished
SRR17060579452,075271.2M162.5Mb2022-03-01

ID:
18126743

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