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SRX13466641: WGS of Erwinia amylovora
1 ILLUMINA (Illumina NovaSeq 6000) run: 2.3M spots, 689.7M bases, 235.8Mb downloads

Design: DNA was extracted according to the Nucleospin Plant II protocol (Macherey-Nagel GmbH & Co. KG, Germany) starting from 250 l aqueous solution of pure culture. The DNA was eluted with 70 l H2O. The extracted DNA has been used to construct the next generation sequencing libraries, starting from 100ng each and using the Kapa Hyperplus library kit (Roche) combined with the Kapa Unique Dual Index adapters (Roche) following the manufacturers instruction. The pooled library was quantified by qPCR and the fragment insertion size of 500bp was evaluated on the Agilent 2200 TapeStation. The final library was sequenced at IGA Technology on NovaSeq 6000 in paired-end 150 mode.
Submitted by: Fondazione Edmund Mach
Study: Erwinia amylovora from Northern Italy (2005-2020)
PRJNA791304 • SRP351904 • All experiments • All runs
show Abstracthide Abstract
Whole genome sequencing of 82 isolates of E. Amylovora collected from different hosts in the Italian region of Trentino between 2005 and 2020.
Sample:
SAMN24274102 • SRS11363932 • All experiments • All runs
Organism: Erwinia amylovora
Library:
Name: 57696_ID1772_34-Ea_15_04_S74_L001
Instrument: Illumina NovaSeq 6000
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 2.3M spots, 689.7M bases, 235.8Mb
Run# of Spots# of BasesSizePublished
SRR172893502,299,092689.7M235.8Mb2022-04-26

ID:
18658509

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