Illumina MiSeq paired end sequencing - SRA

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ERX7018963: Illumina MiSeq paired end sequencing
1 ILLUMINA (Illumina MiSeq) run: 364,306 spots, 219.3M bases, 142.7Mb downloads

Submitted by: Max Planck Institute for evolutionary biology

Study: The effects of sequence length and composition of random sequence peptides on the growth of E. coli cells

PRJEB48999 • ERP133444 • All experiments • All runs

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Amplicon sequencing of random ORF library in E. coli. 5 replicates of the library were sampled every 3 hours for 4 time points. Files follow the naming convention: EXP9-T[sampling time point]-[replicate number]_S[#]_L001_R[1/2]_001.fastq.gz.

Sample: Amplicon sequencing of random sequence library in E. coli

SAMEA11348331 • ERS8993531 • All experiments • All runs

Organism: Escherichia coli str. K-12 substr. DH10B

Library:

Name: T4_1

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: OTHER

Selection: PCR

Layout: PAIRED

Construction protocol: Plasmid DNA was purified from E. coli grown in liquid media for 3 hours (37 ï¿½C, 250 rpm) using QIAGEN kit. Specific primers were used to amplify the pool of random sequence inserts.

Runs: 1 run, 364,306 spots, 219.3M bases, 142.7Mb

Run	# of Spots	# of Bases	Size	Published
ERR7448209	364,306	219.3M	142.7Mb	2022-01-11

ID:: 19071693

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