SRX18469716: WGS of FSL J3-0059
1 ILLUMINA (NextSeq 550) run: 1.3M spots, 392.7M bases, 162Mb downloads
Design: The genomic DNA was extracted by using QIAamp DNA Mini Kit (Qiagen, Valencia, CA) according to the manufacturers instruction, which was modified to include an additional 45 min lysis step. Briefly, cultures grown overnight in BHI broth at 32C were pelleted by centrifugation and subsequently lysed with 20 L of proteinase K (20 mg/mL) by incubation for 45 min at 56C. DNA was eluted twice in 50-L volumes of 10 mM Tris-HCl (pH 8.0), and DNA purity was assessed using a Nanodrop spectrophotometer (Thermo Fischer Scientific, Waltham, MA). Libraries were prepared using Nextera XT DNA Library Preparation Kit (Illumina, San Diego). Samples were pooled and genomic DNA was sequenced in NextSeq 550 with 150-bp pair-end reads.
Submitted by: Cornell University
Study:
Klebsiella and Rahnella sp. isolates associated with ropy defect in milkshow Abstracthide AbstractWhole genome sequencing of Rahnella and Klebsiella sp. isolates associated with ropy defect of milk.
Library:
Name: FSL J3-0059
Instrument: NextSeq 550
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
1 run, 1.3M spots, 392.7M bases, 162Mb