DNA-Seq of Rouxiella badensis: DSM 100043 - SRA

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SRX19145819: DNA-Seq of Rouxiella badensis: DSM 100043
1 OXFORD_NANOPORE (GridION) run: 426,035 spots, 2.3G bases, 1.9Gb downloads

Design: A Single colony was grown overnight in PD broth at 28C with shaking at 200 rpm. The culture was then spun down and the pellet was used for DNA extraction through Promega DNA extraction kit. After checking the quality of the DNA it was used for long read Oxford Nanopore. The long read sequencing was generated by Garvan Institute using an Oxford Nanopore GridION platform with FLO-MIN106 flow cell. The SQK-LSK109 kit was used without shearing and the reads were called and quality controlled during sequencing using MinKNOW version 20.06.17 and Guppy version 4.0.11. This generated total of 268,783 reads.

Submitted by: Charles Sturt University

Study: Complete genome sequence of an Australian strain of Rouxiella badensis DAR84756 and resolution of the R. badensis DSM100043 and R. chamberiensis DSM28324 genomes

PRJNA909035 • SRP418790 • All experiments • All runs

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In this research we announce the complete assembly of a new Australian strain of the Gram-negative bacterium R. badensis DAR84756 and the complete assembly of the German type-strains, R. badensis DSM100043T and R. chamberiensis DSM28324T. The genomes were resolved using ONT long DNA reads and Flye assembler with a mean coverage of over 400x for each genome.

Sample: White to yellow colonies

SAMN32064794 • SRS16558374 • All experiments • All runs

Organism: Rouxiella badensis

Library:

Name: Rouxiella badensis DSM 10043

Instrument: GridION

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 426,035 spots, 2.3G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR23196962	426,035	2.3G	1.9Gb	2023-01-24

ID:: 26341788

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