Macoma balthica Illumina MiSeq mitogenome sequencing, raw paired read... - SRA

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SRX810049: Macoma balthica Illumina MiSeq mitogenome sequencing, raw paired reads, library W20
1 ILLUMINA (Illumina MiSeq) run: 1.3M spots, 641M bases, 319.1Mb downloads

Design: Total genomic DNA was extracted from foot muscle tissue using the DneasyTM Tissue Kit (Qiagen, Germany) following the manufacturer's protocol and stored at -20 °C until further analyses. Mitogenomes were enriched using long-range PCR. Libraries were prepared using the TruSeq DNA Sample Prep Kits v2 and TruSeq Universal Adapters (Illumina, San Diego, CA, USA). A TruSeq Universal Adapter was used for each DNA library in order to separate reads from different individuals after DNA sequencing. Library sizes were checked on BioAnalyzer chips (Agilent Technologies, Santa Clara, CA, USA). Paired-end library sequencing was carried out on the Illumina MiSeq plateform (2 x 250 bp chemistry) at the GeT-PlaGe lab (GenoToul, Toulouse, France).

Submitted by: University of La Rochelle

Study: Macoma balthica Mitogenome sequencing

PRJNA270311 • SRP051152 • All experiments • All runs

Sample: Foot tissue from field-collected specimen

SAMN03265463 • SRS791274 • All experiments • All runs

Organism: Limecola balthica rubra

Library:

Name: W20

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Spot descriptor:

1 forward251 reverse

Runs: 1 run, 1.3M spots, 641M bases, 319.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR1713369	1,281,928	641M	319.1Mb	2014-12-15

ID:: 1159737

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