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SRX20648986: GSM7471644: Trichoplax adhaerens H3K4me2 ChIP-seq; Trichoplax adhaerens; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 21.1M spots, 1.6G bases, 641.7Mb downloads

External Id: GSM7471644_r1
Submitted by: Sebe-Pedros lab, Systems Biology, Centre for Genomic Regulation
Study: Stepwise emergence of the neuronal gene expression program in early animal evolution
show Abstracthide Abstract
We performed single-cell transcriptome analysis (using 10x) of four placozoan species (Trichoplax adhaerens, Trichoplax sp. H2, Cladtertia collaboinventa, Hoilungia hongkongensis). Additionally we performed bulk ATAC-seq experiments and anti-H3K4me2/anti-H3K4me3 ChIP-seq experiments the same species. Overall design: Adult specimens were fixed, dissociated and pooled before sorting single-cells for scRNAseq. Some samples were mixed (cross-species or cross-treatments) and processed together. Instructions for demultiplexing cells are provided. Please note that the processed data for the scRNA-seq samples are generated from multiple samples (as indicated in the corresponding sample descriptionfield) and thus is linked to the Series records.
Sample: Trichoplax adhaerens H3K4me2 ChIP-seq
SAMN35687512 • SRS17947116 • All experiments • All runs
Library:
Name: GSM7471644
Instrument: NextSeq 500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: ChIP-seq was performed as described in 10.1038/s41559-018-0575-6. Adult specimens were fixed, dissociated and pooled before sorting single-cells for scRNAseq. The same procedure was followed for chromatin profiling experiments, except that cells were used fresh for ATAC-seq and fixed with 1% formaldehyde (10 min) for ChIP-seq
Runs: 1 run, 21.1M spots, 1.6G bases, 641.7Mb
Run# of Spots# of BasesSizePublished
SRR2488638221,146,3901.6G641.7Mb2023-09-21

ID:
28078223

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