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SRX20648970: GSM7471628: Mixed scRNA-seq Trichoplax adhaerens and Cladtertia collaboinventa 1; Trichoplax adhaerens; Hoilungia sp. H23; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 239.4M spots, 20.1G bases, 7.7Gb downloads

External Id: GSM7471628_r1
Submitted by: Sebe-Pedros lab, Systems Biology, Centre for Genomic Regulation
Study: Stepwise emergence of the neuronal gene expression program in early animal evolution
PRJNA982113 • SRP442048 • All experiments • All runs
show Abstracthide Abstract
We performed single-cell transcriptome analysis (using 10x) of four placozoan species (Trichoplax adhaerens, Trichoplax sp. H2, Cladtertia collaboinventa, Hoilungia hongkongensis). Additionally we performed bulk ATAC-seq experiments and anti-H3K4me2/anti-H3K4me3 ChIP-seq experiments the same species. Overall design: Adult specimens were fixed, dissociated and pooled before sorting single-cells for scRNAseq. Some samples were mixed (cross-species or cross-treatments) and processed together. Instructions for demultiplexing cells are provided. Please note that the processed data for the scRNA-seq samples are generated from multiple samples (as indicated in the corresponding sample descriptionfield) and thus is linked to the Series records.
Sample: Mixed scRNA-seq Trichoplax adhaerens and Cladtertia collaboinventa 1
SAMN35687528 • SRS17947099 • All experiments • All runs
Organism: Trichoplax adhaerens
Library:
Name: GSM7471628
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells were fixed using the ACME protocol (10.1186/s13059-021-02302-5), FACS sorted, and processed with the 10X Genomics Chromium Single Cell 3' scRNAseq v3.1 kit. Adult specimens were fixed, dissociated and pooled before sorting single-cells for scRNAseq. The same procedure was followed for chromatin profiling experiments, except that cells were used fresh for ATAC-seq and fixed with 1% formaldehyde (10 min) for ChIP-seq
Runs: 1 run, 239.4M spots, 20.1G bases, 7.7Gb
Run# of Spots# of BasesSizePublished
SRR24886413239,409,60120.1G7.7Gb2023-09-21

ID:
28078207

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