show Abstracthide AbstractPetroleum hydrocarbons are recalcitrant contaminants, which has caused most serious environmental problems. Acinetobacter calcoaceticus Aca13 was isolated from petroleum polluted soil for petroleum biodegradation. Hexadecane and naphthalene were used to incubate with Acinetobacter calcoaceticus Aca13. After incubation, the whole transcriptome was obtained from treated groups and control groups, and then used for RNA sequence and analysis. Obtained data in this project will help us understand the biodegradation mechanism of hexadecane and naphthalene, and will be helpful for the bioremediation of petroleum hydrocarbons. Overall design: 3 Groups: Hexadecane group (named as AcaH1, AcaH2, AcaH3, respectively), naphthalene group (named as AcaN1, AcaN2, AcaN3, respectively) and control group (named as Aca1, Aca2, Aca3, respectively). Each group has three replicates.