SRX21345949: Thermus thermophilus HB27 deltaCmr4: escaper library replicate 2
1 OXFORD_NANOPORE (MinION) run: 51,481 spots, 58.1M bases, 52.8Mb downloads
Design: Thermus thermophilus HB27 strain with knockdown of the Type IIIB CRISPR-Cas system. "Escapers" colonies that lack IIIA system as well occur via recombination by CRISPR cassettes with deletion of the megaplasmid fragment. Amplicon sequencing of potentially recombinant CRISPR cassettes.
Submitted by: Skolkovo Institute of Science and Technology
Study:
Interference Requirements of Type III CRISPR-Cas Systems from Thermus thermophilusshow Abstracthide AbstractThe project aims to understand required features of the target sequence that cause an activity of Type III CRISPR-Cas system in bacterium Thermus thermophilus.
Sample:
escaper library from dCmr4 strain, replicate 2Library:
Name: deltaCmr_rep2
Instrument: MinION
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: SINGLE
Runs:
1 run, 51,481 spots, 58.1M bases, 52.8Mb