9MG-resistant Dd2 clone F3 experiment 1 - SRA

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SRX22119095: 9MG-resistant Dd2 clone F3 experiment 1
1 ILLUMINA (Illumina NovaSeq 6000) run: 4.6M spots, 1.4G bases, 449.8Mb downloads

Design: Parasites were liberated from host cells by saponin lysis. Genomic DNA was extracted by cell lysis with SDS/proteinase K, followed by phenol:chloroform extraction and ethanol precipitation as previously described. Genomic DNA pellets were resuspended in 10 mM Tris, 1 mM EDTA and assessed by agarose gel electrophoresis and Nanodrop spectrometry. The purified genomic DNA samples were shipped on dry ice to NovogeneAIT (Singapore) for whole-genome sequencing service.

Submitted by: National Center for Genetic Engineering and Biotechnology (BIOTEC), NSTDA

Study: 9-methoxystrobilurin-G induced resistance

PRJNA1029235 • SRP466905 • All experiments • All runs

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The natural product 9-methoxystrobilurin-G (9MG) from Favolaschia spp basidiomycetes is a potent and selective antimalarial. The mechanism of action of 9MG is unknown, but structurally related strobilurins from other fungal species are known to inhibit mitochondrial cytochrome b (CYTB) complex III. We induced 9MG resistance in Plasmodium falciparum laboratory strains 3D7 and Dd2 and identified mutated genes associated with resistance by genome sequencing.

Sample: 9MG-resistant Dd2 clone F3 experiment 1

SAMN37864593 • SRS19183073 • All experiments • All runs

Organism: Plasmodium falciparum Dd2

Library:

Name: Dd2_1_F3

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 4.6M spots, 1.4G bases, 449.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR26413772	4,563,332	1.4G	449.8Mb	2024-08-30

ID:: 30065577

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