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SRX23390107: Whole genome sequencing of V. fischeri strain ES114
1 ILLUMINA (NextSeq 500) run: 5M spots, 1.5G bases, 619.7Mb downloads

Design: Genomic DNA from V. fischeri strains was extracted using the Invitrogen PureLink Genomic DNA Mini Kit. DNA was sonicated to generate fragments of approximately 500 bp, and gDNA libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina, which includes end-repair, adaptor ligation, and addition of index primers. Sequencing was performed by the University of Georgia Genomics and Bioinformatics Coreon an Illumina NextSeq500 instrument.
Submitted by: University of Georgia
Study: Vibrio fischeri D-glutamate suppressor generation and characterization
show Abstracthide Abstract
This study used D-glutamate auxotrophic mutants of Vibrio fischeri to study the evolution of peptidoglycan biosynthesis and structure. Suppressor mutants were generated by growing an auxotrophic strain without necessary exogenous D-glutamate. Mutants were then characterized and genome sequenced, in order to determine the mechanism of suppression.
Sample:
SAMN39609062 • SRS20250971 • All experiments • All runs
Library:
Name: ES114
Instrument: NextSeq 500
Strategy: WGS
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 5M spots, 1.5G bases, 619.7Mb
Run# of Spots# of BasesSizePublished
SRR277241844,992,8851.5G619.7Mb2024-02-01

ID:
31610660

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