Whole genome sequencing of Vibrio fischeri strain derived from ES114 - SRA

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SRX23668395: Whole genome sequencing of Vibrio fischeri strain derived from ES114
1 ILLUMINA (NextSeq 2000) run: 1.3M spots, 380.5M bases, 130.1Mb downloads

Design: Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.

Submitted by: University of Georgia

Study: Vibrio fischeri D-alanine suppressor generation and characterization

PRJNA1078398 • SRP490497 • All experiments • All runs

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This study used D-alanine auxotrophic mutants of Vibrio fischeri to study the evolution of peptidoglycan biosynthesis and structure. Suppressor mutants were generated by growing an auxotrophic strain without necessary exogenous D-alanine. Mutants were then characterized and genome sequenced, in order to determine the mechanism of suppression.

Sample:

SAMN39999379 • SRS20500242 • All experiments • All runs

Organism: Aliivibrio fischeri

Library:

Name: MC6S01

Instrument: NextSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 1.3M spots, 380.5M bases, 130.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR28016261	1,279,224	380.5M	130.1Mb	2024-02-20

ID:: 31951812

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