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SRX23868035: Paired-end Illumina sequencing of C. botulinum PB0612A isolated from peanut butter from adult botulism case
1 ILLUMINA (Illumina MiSeq) run: 688,210 spots, 374.7M bases, 236.6Mb downloads

Design: Single colony grown 24h at 35 degrees in 25mL TPGY. Pellet resuspended in 1mL of gelatin phosphate buffer and 1mL of 2 Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAse A treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry
Submitted by: Health Canada
Study: Clostridium botulinum Genome sequencing and assembly
show Abstracthide Abstract
Clostridium botulinum genome sequencing and assembly of clinical and food isolates from an adult botulism case from 2006 and retail peanut butter in 2007.
Sample: C. botulinum isolate PB0612A, BoNT/A; Isolated from opened peanut butter from 2006 adult botulism case.
SAMN40298543 • SRS20686918 • All experiments • All runs
Library:
Name: BMH-2021-001129
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 688,210 spots, 374.7M bases, 236.6Mb
Run# of Spots# of BasesSizePublished
SRR28257552688,210374.7M236.6Mb2024-06-27

ID:
32172274

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