RNA-Seq Analysis of Colonial Phase Variants of V. cholerae SD2 - SRA

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SRX1225091: RNA-Seq Analysis of Colonial Phase Variants of V. cholerae SD2
1 ILLUMINA (Illumina HiSeq 2000) run: 22.2M spots, 2.2G bases, 860.3Mb downloads

Design: RNA samples were chemically fragmented to 400-500 ntds. To create 5´ to 3´ strand-specific cDNA libraries, a TruSeq Stranded Sample Preparation kit was used, followed by gel extraction to select fragments with a minimum size of 80 ntds. Fragments were barcoded and multiplexed with other samples from this study on a single lane of an Illumina HiSeq2000 for 101 cycles. From RNA population of 80-500 ntds, 100 ntds were sequenced from randomly selected ends using the TruSeq SBS Sequencing Kit Version 3 and demultiplexed with Casava 1.8.2.

Submitted by: Louisiana State University

Study: Vibrio cholerae O1 biovar El Tor str. N16961 Transcriptome or Gene expression

PRJNA295073 • SRP063467 • All experiments • All runs

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Vibrio cholerae switches between smooth and rugose colony variants in a reversible process known as phase variation. In this study, we analyzed the transcriptomes of smooth and rugose colony variants in order to investigate differences in gene expression related to this type of phase variation.

Sample: N16961SD

SAMN04040919 • SRS1064982 • All experiments • All runs

Organism: Vibrio cholerae O1 biovar El Tor str. N16961

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Spot descriptor:

1 forward

Pipeline: show...hide...

Program	Version
BowTie2	2.0.0-beta5

Runs: 1 run, 22.2M spots, 2.2G bases, 860.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR2302175	22,205,991	2.2G	860.3Mb	2016-11-04

ID:: 1771354

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