show Abstracthide AbstractRumen fermentation is a major contributor to global warming through the emission of greenhouse gases. It is the second largest contributor to methane production, with anthropogenic causes in the first place. The importance of reducing methane emissions from the livestock sector has become a priority, due to the increasing demand for livestock and the ecological impact this entails. Therefore, to mitigate this problem, alternatives in terms of livestock feeding are being developed to try to reduce greenhouse gas emissions. Previously, the use of Moringa oleifera as a supplement in livestock diets has been shown to reduce methane and carbon dioxide emissions. The aim of this study is to clarify, using metatranscriptomic analysis technology, the effects of Moringa oleifera on methane metabolism in rumen enviroment.